- Completed PACE training program- Independently monitor clinical sites- Conduct qualification, initiation, routine monitoring, and close out visits- Develop and maintain site relationships to facilitate effective monitoring and oversight- Ensure trials are being conducted per protocol and GCP

- Performed QA at a large beverage production and packaging facility while upholding GMP and GLP- Managed sample collection and analysis for each batch produced at the facility- Performed both microbiological and chemical analysis of all products

- Developed a method in python for predicting interactions in large protein complexes utilizing AlphaFold Multimer, resulting in the ability to make predictions that were previously impossible- Creating a machine learning model to evaluate computer predicted protein interfaces- Established and optimized a new protein production pipeline and developed reagents for production

- Investigated the structure of the ADAM family of proteins- Creating a “knockout” cell line absent of our protein of interest- Optimized protein expression in a mammalian cell culture system- Created protein expression viruses for insect cell protein production- Developed protein-specific purification strategies- Screened and optimized crystallization conditions- Designed domain specificity experiments using structural dataPresentations:Shaw, NR; Seegar, TCM. Expression and purification of the ADAM10 prodomain for structure determination. Poster presented at: Ohio Physiological Conference; September 2021; Cleveland, OH.Shaw, NR; Seegar, TCM. ADAM10 prodomain purification and crystallization. Poster presented at: Summer Undergraduate Research Fellowship Presentations, University of Cincinnati, College of Medicine; July 2021; Cincinnati, OH.Shaw, NR; Seegar, TCM. Study of inflammatory enzymes in human development and disease. Poster presented at: University of Cincinnati, Undergraduate Scholarly Showcase; April 2021; Cincinnati, OH.

- Investigated neural stem cell migration and differentiation- Analyzed 3 channel, 5 layer confocal images using Fiji with a custom macro- Improved image analysis macro to reduce analysis time by 10% saving approximately 100 hoursPublication:Olig2 defines a subset of neural stem cells that produce specific olfactory bulb interneuron subtypes in the subventricular zone of adult micehttps://doi.org/10.1242/dev.200028

- Cared for customer needs on an individual basis- Stocked ingredients necessary for the preparation of ice cream products- Worked with a team to deliver wholesale ice cream