Student Researcher
- Investigated the structure of the ADAM family of proteins- Creating a “knockout” cell line absent of our protein of interest- Optimized protein expression in a mammalian cell culture system- Created protein expression viruses for insect cell protein production- Developed protein-specific purification strategies- Screened and optimized crystallization conditions- Designed domain specificity experiments using structural dataPresentations:Shaw, NR; Seegar, TCM. Expression and purification of the ADAM10 prodomain for structure determination. Poster presented at: Ohio Physiological Conference; September 2021; Cleveland, OH.Shaw, NR; Seegar, TCM. ADAM10 prodomain purification and crystallization. Poster presented at: Summer Undergraduate Research Fellowship Presentations, University of Cincinnati, College of Medicine; July 2021; Cincinnati, OH.Shaw, NR; Seegar, TCM. Study of inflammatory enzymes in human development and disease. Poster presented at: University of Cincinnati, Undergraduate Scholarly Showcase; April 2021; Cincinnati, OH.