Processing samples from animal studies including liver, brain, ocular and peripheral tissues in 96 well plate format● Recorded experimental work using Benchling as ELN, cryostat dissection, homogenization and trizol DNA/RNA extraction, reverse transcription with unique barcodes, qPCR, pre-indexing and pooling samples and finally indexing with unique Illumina pairs and next-gen sequencing amplicons for computational analysis● Process development work including improved homogenization methods, alternate indexing primers and methods, new reverse transcription methods and automated DNA/RNA extraction with SPRI beads and KingfisherAAV vector production of low throughput (1-4 variants) and medium throughput libraries (50-100 variants)● HEK293 cell culture in hyperflasks, transfection of libraries separately (10-25 sequences per round, 100+ total), cell lysis, trans-flow filtration, iodixanol ultracentrifugation, buffer exchange and titering● Standard QC methods including ddPCR, endotoxin, sterility, silver stain protein gels, and NGS to see individual sequence’s productivity ● Vector production of a test article of a novel sequence for a CNS study and performed all subsequent tissue processing, resulting in data shared at ASGCT demonstrating effectiveness of bCap1, with 50-100x pan-brain transduction relative to wildtype, ~5-10% total neurons

Developing HT protein quantification assays for S.cerevisiae and P.pastoris for use in ML algorithms improving codon-optimization and amino acid sequence● Engineered P.pastoris strains and developed HiBit assay for secreted protein quantification● Engineered S.cerevisiae strains with split FP reporters, developing plate reader and FACS assaysScale up commodity chemical titer to 100g/L in an pre-engineered E.coli strain.● Screened hundreds of strains with combinatorial pathway plasmids using quantitative small scale(QSS) fermentation assays and analyzing mass-spectrometry data● NGS and data analysis of fermentation runs in Amber250sDeveloping HT E.coli genome engineering pipeline for Ginkgo Foundry● Wrote the R&D proposal, co-wrote the TDP, selected to move forward out of 40 proposals● Automated SOP including transformation, nuclease induction and counter-selectionTool development in an undomesticated methylotrophic strain● Ran electroporation and conjugation DOEs for plasmid transformation● Optimized antibiotics, media, growth conditions, cryostocking and NGS submission conditionsSynthetic methylotrophy in prokaryote to produce a commodity chemical monomer● Engineered semi-methylotrophs via RuMP pathway and rebalanced central carbon metabolism● In-vitro enzyme screens, discovering diverted pathway flux and identifying knockout targets

Researching under Dr. Ted Meigs how lipidation and polybasic motifs play a role in signal strength of the oncogenic G protein GA13. This includes making chimeric proteins via PCR mutagenesis, SRE-luciferase assays, effector protein pulldowns, western blots, a click chemistry assay I designed and eventually, flow cytometry.

Internship at F(x) Immune. I was introduced to flow cytometry here and ran Opsonophagocytic assays. I was also present for business meetings and learned some basic business practices.