Nick Doperalski, Ms Email & Phone Number

Gaithersburg, Maryland

Gaithersburg, Maryland

Richmond, Virginia Area

Promoted several times based on exemplary service and results to maintain program management responsibilities for multiple concurrent clients, including contract administration, project logistics, operations, quality control and assurance and meeting FDA and EMA regulatory guidance. Currently serve as R&D Program Manager for two clients.• Successfully managed a team of up to five scientists and analysts in bioanalytical method development and validation in a GLP laboratory environment… Show more Promoted several times based on exemplary service and results to maintain program management responsibilities for multiple concurrent clients, including contract administration, project logistics, operations, quality control and assurance and meeting FDA and EMA regulatory guidance. Currently serve as R&D Program Manager for two clients.• Successfully managed a team of up to five scientists and analysts in bioanalytical method development and validation in a GLP laboratory environment for multiple clients• Broad experience developing and validating pharmacokinetic, immunogenicity and enzyme activity assays for novel drug products, biosimilars / bioequivalents, and biomarkers• Understanding of FDA and EMA regulatory guidance regarding validation of immunoassays to support pre-clinical and clinical pharmaceutical trials• Utilizing electrochemiluminescence (ECL/MSD), enzyme-linked immunosorbent assay (ELISA), Gyrolab (Gyros), fluorescence (Wallac), and radioimmunoassay (RIA) technologies Show less

Richmond, Virginia Area

Richmond, Virginia Area

Richmond, Virginia Area

Gainesville, Florida Area

Examined physiological and molecular effects of persistent agricultural pesticides in piscine species• Molecular cloning of gene sequences from atypical model species• Assessment of gene expression in biological samples using quantitative-PCR (qPCR)• Gas chromatography-mass spectrometry (GC-MS) techniques and methods• Extraction techniques from biological samples, including solid-phase extraction chromatography (SPE) and gel-permeation chromatography (GPC)• In vitro… Show more Examined physiological and molecular effects of persistent agricultural pesticides in piscine species• Molecular cloning of gene sequences from atypical model species• Assessment of gene expression in biological samples using quantitative-PCR (qPCR)• Gas chromatography-mass spectrometry (GC-MS) techniques and methods• Extraction techniques from biological samples, including solid-phase extraction chromatography (SPE) and gel-permeation chromatography (GPC)• In vitro tissue explant culture and exposure• Immunoassays including RIA, ELISA• Immunohistochemistry (IHC)• Interpretation of proteomic data sets• Statistical analysis and presentation of experimental results• Preparation of manuscripts for publication in peer-reviewed scientific journals• Management of laboratory Show less

Acquired Skills:• Ex vivo tissue culturing techniques• Radioimmunoassay (RIA) and enzyme immunoassay (EIA) optimization• Organic solvent and solid-phase chromatography (SPE) extraction techniques from plasma and animal tissue• Gas chromatography-mass spectrometry (GC-MS) determination of organic compound concentration in tissue and biomass• Gene cloning techniques in an atypical model species (Micropterus salmoides) including: o Use of NCBI BLAST technology o… Show more Acquired Skills:• Ex vivo tissue culturing techniques• Radioimmunoassay (RIA) and enzyme immunoassay (EIA) optimization• Organic solvent and solid-phase chromatography (SPE) extraction techniques from plasma and animal tissue• Gas chromatography-mass spectrometry (GC-MS) determination of organic compound concentration in tissue and biomass• Gene cloning techniques in an atypical model species (Micropterus salmoides) including: o Use of NCBI BLAST technology o Sequence alignment techniques utilizing Clustal software o Design of custom degenerate PCR primers o Evaluation of PCR product via agarose gel electrophoresis o Bacterial transfection, blue/white (x-gal) selection, colony PCR o Plasmid isolation/preparation for insert sequencing o 3’ and 5’ RACE technique• Handling and tissue dissection of fish species including largemouth bass, zebrafish (Danio rerio), and fathead minnow (Pimephales promelas)• Enhancement of statistical evaluation skills and application to diverse experimental data Show less

Gainesville, Florida Area

Managed a federally-funded research laboratory, including planning and executing experiments, maintaining inventory and instrumentation, assisting with grant applications, compiling data and ensuring compliance with Environment Health and Safety regulations.• Designed and executed studies aimed at furthering the understanding of spinal cord injury and development of therapeutic strategies• Collected, analyzed, and interpreted experimental data and assisted in preparation of written… Show more Managed a federally-funded research laboratory, including planning and executing experiments, maintaining inventory and instrumentation, assisting with grant applications, compiling data and ensuring compliance with Environment Health and Safety regulations.• Designed and executed studies aimed at furthering the understanding of spinal cord injury and development of therapeutic strategies• Collected, analyzed, and interpreted experimental data and assisted in preparation of written manuscripts for publication in peer-reviewed scientific journals• Presentation of findings at regional and national professional conferences• Numerous biochemical assays, physiological evaluation, and surgical procedures• Management of laboratory Show less

• Assisted in experiments investigating neurophysiology of the rat, specifically addressing the mechanisms underlying respiratory neuroplasticity• Designed and maintained a quantitative-PCR laboratory as well as developed experimental protocols and standard operating procedures• Cultured N9, BV-2, and HT-22 murine cell lines• Performed protein analysis using immunoblot (Western), ELISA, immunohistochemistry, and BCA assay

Master Of Science, Toxicology

Activities and Societies: Geosyntec Graduate Student Platform Award, SESETAC Conference, 2009; Veterinary Graduate Student Association.

Bachelor Of Science, Biology, Chemistry, Computer Information Systems

Activities and Societies: Awards: R. F. Wilde Outstanding Biology Major Award (2001, University of Wisconsin); Biology Faculty Student.