Nicolas Acosta Email and Phone Number

- Developed Algorithm to co-register diffraction limited light microscopy images from Partial Wave Spectroscopy Microscopy to super resolution single molecule localization techniques such as STORM. - Developed robust pipeline to perform single molecule localization microscopy on three nuclear targets to investigate the distribution of histone modifications and RNA Polymerase II with a sub-15 nm precision. - Co-developed clustering algorithm to explore joint distributions of histone post translational modifications from point-cloud localization datasets. Explored use of point-cloud based and image based analysis for super resolution image reconstructions. - Developed and tested GUI Software to implement Neural Network Automatic Nuclear segmentation with in house Partial Wave Spectroscopy Analysis software (Python/JavaScript). Released on PyPI.- Developed Microfluidic Shear Cell culture device to monitor dynamic effects of fluid shear stress on chromatin packing to understand how mechanical characteristics of the nucleus pre-determine success of metastatic extravasation

-Aided in the optimization of a microfludic chip based immunoassay used to diagnose Acute Kidney Injury by measuring the concentrations of Neutrophil Gelatinase Associated-Lipocalin in patients urine-Produced and tested microfluidic chips with patient urine samples of varying NGAL concentrations in order to determine the assay’s ability to produce accurate signal-Investigated the use of antibody stabilizers to improve assay generated signals in order to ensure statistical difference between analyte groups of varying concentrations- Developed Python Program to automate data collection and generate Microsoft Excel files to display the optical density from the immunoassay test. - Aiding current development of microfluidic device to detect antimicrobial resistance in Neisseria gonorrhoeae- Designed and performed CFD analysis on a fluidic device being used for automated gram staining in SolidWorks and Ansys

- Selected from a pool of thousands of candidates to work closely with leaders of RippleMatch's Leadership Team - Leveraged various growth strategies and tools including social media, email marketing, presentations, and peer and faculty member networking to grow the user base and awareness on campus- Strategically assessed growth and performance metrics to improve, change and/or help design new growth strategies

- Oversees organization that aims to connect Latinx Undergraduates to industry professionals with relevant experiences.- Organizes yearly networking event, Café con Leche, that brings Latinx professionals from a variety of fields to Columbia in order to connect them with undergraduates. - Co-started a STEM tutoring program with Double Discovery Center for High School students in the Columbia area.

- Used CRISPR-cas9 system to analyze the regulatory relationship between two important genes, tra-1 and daf-12 in the C.elegans model organism- Developed two CRISPR modified lines in order to analyze the role of these transcription factors in the development of a sexually dimorphic nervous system

Three week intensive course under Professor Donna Lizzote concerning research methods an laboratory techniques in Biomedical Fields• Proficient in Gel Electrophoresis, Western and Southern Blotting• Ran Experiments on the use of Restriction Enzymes to cut lambda DNA under various conditions• Explored possibility of using TET Enzymes to de-methylate strands of bacterial DNA and the use of Plasmids to transfer genetic material to other bacteria

Six week intensive program under Professor Robert Dell concerning sustainable Energy and Green sources. • Developed Solar Powered Desalination system for applications in areas with minimal access to potable water• Learned Basic Design process • Gained Proficiency in Solid Works an Excel