Nicole Capozzi Email & Phone Number

• Initiate and maintain embryonic cell cultures of sea urchin cells • Count cells using a hemocytometer and track growth of cultures • Image cells with both brightfield and fluorescence microscopy • Conduct experiments with Lonza and BioRad systems • Validate genetic identity of cells using Polymerase Chain Reaction (PCR) and Illumina sequencing

Katz Lab

• Researched marine microbial eukaryotes using metatranscriptomics and metabarcoding • Analyzed gene trees created using in-house PhyloToL pipeline• Built phylogenetic trees using in-house Miseq pipeline• Conducted PR2 read mapping and analyzed results in R• Wrote thesis paper on results of the study and defended project in final honors presentation

Italian Studies Department

• Graded student quizzes for Savoring Italy course• Assisted with uploading grades into Moodle grade book• Answered student questions

Katz Lab

• Imaged samples with Scanning Electron Microscope FEI Quanta 450• Performed Total RNA Isolation on samples of seawater to determine ciliate presence or absence• Quantified RNA with Quibit 3.0 Fluorometer

Florence, Tuscany, Italy

• Cultured T-cells in preparation for clinical trials of a developed melanoma treatment• Observed cells under microscope to determine presence of contamination and health of cells• Split and froze cultures as necessary to prepare for clinical trials• Prepared culture medium and extracted buffy coat from blood samples for use in cell stimulation

Rwright Lab

• Conducted experiment on impact of heat and cold stress on anemone Exaiptasia diaphana• Performed tissue extraction on anemone samples to isolate host and symbiont fractions• Used hemocytometer to determine symbiont count for symbiont fractions• Measured carbohydrate content in host and symbiont fractions• Measured peroxide content in host and algal water samples• Imaged anemones on Zeiss Axio Imager M2 Light Microscope • Used R to create figures and tables representing experimental results• Documented results of heat and cold stress experiment

Rwright Lab

• Provided care for symbiotic and aposymbiotic sea anemones• Contributed to cloning experiments and prepared for anemone spawning• Conducted experiment about the relationship between the cloning rate of anemones and the presence of sediment and/or mangroves

Boston University Clinical Testing Laboratory

• Completed viral inactivation• Prepared reagents for extraction and purification of RNA samples• Manually assigned and pipetted samples to complete pooling process• Prepared master mix and ran qPCR procedure to determine presence/absence of SARS-CoV-2• Processed samples through receipt, sample processing, and storage according to lab protocols• Operated semi-automated Hamilton robots• Worked in compliance with written SOPs and CLIA regulations

Bachelor'S Degree, Biological Sciences And Italian Studies Double Major, Gpa: 3.97, Cum Laude With Highest Honors In Biological Sciences

Activities and Societies: Katz Lab - 2022 - 2024, Italian Culture Club (Club President) - 2022 - 2024, Page Turners Book Club (Club.

Non-Degree Student

CAS BI 414 - Ornithology MET AH 216 - Digital Photography

Non-Degree Student

Stat 240 - Introduction to Probability and Statistics NRC 260 - Fish Conservation and Management Chem 111 - General Chemistry

High School Diploma

Activities and Societies: Varsity Ultimate Frisbee - 2016-2019 (Team Captain 2019), Pingree "Pulse" A Capella - 2017-2019, Polar Plunge.

Fish Fraud Forensics Summer Program, Marine Sciences

This was a week long program that covered the topic of fish fraud through an analysis of an unidentified fish tissue sample. The program.

High School Program, Oceanography And Maritime History

This was a three week program through SEA which included two courses: Oceanography and Maritime History. I received the highest ranking.