Nicole Schapiro Email & Phone Number

Pittsburgh, Pa

Drs. Herbert Zeh III, Michael T. Lotze, and Daolin TangManaged large mouse colony, including transgenic breeding and genotyping, orthotopic, subcutaneous and metastatic pancreas tumor models, in vivo imaging, record keeping and all regulatory paperwork for multiple investigators • Managed research samples for four simultaneous clinical trials, including blood and tissue banking, record keeping and coordination with clinical staff and complementary experiments • Worked closely with surgical residents on laboratory rotations for training, support and coordinating complex in vivo and in vitro experiments • Ongoing laboratory maintenance and upkeep of a proper lab space for approximately 12 lab members under five faculty comprised of troubleshooting experiments, training new employees, safety checks, equipment repair and supply ordering • Updating and maintaining IACUC and recombinant DNA protocols for multiple projects • Evaluate lab safety techniques and prepare for annual laboratory inspections • Execute and analyze independent in vitro and in vivo experiments utilizing techniques such as SDSPAGE, dot blots, protein and cell extraction from tissue, cryosectioning and tissue processing for histological staining, aseptic cell culture techniques of tumor and primary cell lines, flow cytometry • Provide research updates during weekly lab meetings • Superb record-keeping associated with animal models, in vitro experiments and clinical trials using Word, Excel, Powerpoint • Attended multiple local and international meetings; prepared abstracts and poster presentations

Studied the osteogenesis of adult human mesenchymal stem cells using molecular biology techniques • Exposure to viral transfections • Utilized aseptic cell culture techniques to culture mesenchymal cells • Awarded position for fall semester through Howard Hughes Medical Institute • Presented research at the 2005 American Society for Cell Biology meeting

Introduction to cell culture of normal and transformed human fibroblasts • Independently developed experimental protocols • Learned aseptic techniques, SDS-PAGE, western blots, affinity chromatography columns and fluorescent staining and microscopy

Master Of Science Degree, Bioengineering

Bachelor Of Science, Biomedical Engineering

Bachelor Of Science, Biology

Education record