• Co-led high-priority ocular siRNA project from target selection through preparation of documentation for New Molecule Entity (NME) and coordination with Clinical Development Team• Ensured alignment of multiple functional groups to meet objectives and timelines• Developed assays using transfection and free-uptake to assess siRNA efficacy in multiple cell lines• Coordinated siRNA microarray selectivity analysis pipeline and tool development, in collaboration with colleagues in Oligo Biology and Computational Biology departments• Leveraged public scRNA-seq data sets to identify and characterize target cell populations• Contributed to preparation of one provisional patent• Served as Environmental Health & Safety lead for Oligo Biology Lab

• Helped develop “Negotiation Playbook” project for creation of recommended deal terms repository.• Studied, assessed and summarized licensing deals.• Member of the MBC Biolabs Selection Committee identifying and screening startup companies for incubator space.• Identified new licensing and partnering opportunities and documented these in an internal deals database.• Assessed competitive landscape of oligonucleotide-related technologies.

• Developed 2 novel reporter plasmids to mark early reprogramming populations; contributed to public repository (Addgene)• Mentored 5+ lab members on command-line skills, use of sequencing alignment pipeline, and genomic data analysis and visualization• Led genomic analysis and integration of ChIP-seq data as part of a collaborative team, along with bioinformatics core members including one Biostatistics PhD and one Computational Biology PhD

• Characterized induced (transdifferentiated) cardiomyocytes at multiple stages of cardiac reprogramming, revealing mechanisms that inform therapeutic strategies, resulting in discovery of 8 experimentally validated transcription factor candidates and co-first author publication in Cell Stem Cell• Optimized protocols to succeed with small cell numbers, allowing interrogation of rare cell population• Pioneered use of ATAC-seq in the Srivastava lab and mentored 6 scientists both internally and externally on ATAC-seq experimental procedure and analysis, resulting in co-authorship of publication in Cell• Managed team of up to 5 bioinformaticians and biologists to generate, analyze, integrate genomic data sets

• Completed a computational investigation of miRNA regulation of folate metabolism pathway, identifying putative miRNA “master regulators” and resulting in first author publication in PLoS One• Investigated correlation of genetic variation with folate metabolism and complex birth defects (e.g., spina bifida and cleft palate) using a multiplexed genotyping approach. Results published in BMC Med Genet •

• Assayed targeted mutations of vaccinia virus I3 protein to find the basis of its cytoplasmic localization• Cell culture, cloning, Western blot, microscopyMentor: Paula Traktman

• Designed and carried out project examining the effects of beaver pond morphology on benthic invertebrate communities• Found that beaver pond morphology (depth, tiers, flow dynamics) affects downstream benthic invertebrate community composition• High-altitude site survey, benthic invertebrate capture and identification at the genus levelMentors: Barbara Peckarsky, Angus McIntosh, Matthew Fuller

• Characterized cyanobacteria population composition during primary succession in an abandoned limestone quarry• Field research sample collection, microscopyMentor: Dana Dudle

• Identified species-specific pupation site preferences in native swallowtail butterflies• Caught and reared native swallowtail species• Project design, analysis, paper writingMentor: Wade Hazel