Nicole A. Email & Phone Number

San Diego, California, United States

Develop and validate bioanalytical methods according to current FDA regulations and departmental SOPs for potential small molecule drugs candidates.Complete analysis of clinical biological samples in various matrices within a given time-line in a GLP environment using various sample preparation techniques and analysis by LC/MS/MS. Work closely with colleagues on more complex assays and troubleshooting instrumentation. Train and mentor contractors, graduate and undergraduate interns to ensure proper training in good laboratory techniques, theory and instrumentation while giving them valuable work experience. Maintain the operation of the cell processing lab used to isolate the peripheral blood mononuclear cells (PBMC's) from whole blood. Improved previous cell processing method to save time, money, give cleaner blank matrix and a more user friendly method. Evaluate and give input about clinical protocols for sample collection and processing of PBMCs to ensure that best sample quality possible is received to have more reliable data. Develop and implement an in-house automated cell counting method using DNA florescence tagging for study samples, eliminating human error in cell counts from clinical samples. Improve the LC/MS/MS method for intracellular triphosphate levels by removing the need for ion pairing modifiers from mobile phase and more intense sample preparation method to eliminate the effects of poor sample processing by clinics, leading to more reliable data.Write, issue, and review important documents such as notebooks, analytical methods, validation reports, sample analysis reports, study investigations and SOPs to ensure quality, accuracy and current with industry standards.Give oral presentations within our department and organization about relevant research projects.

Authored SOPs for lyophilizer and CE/UV-Vis system.Trained faculty, staff and research students on proper operating procedures for the lyophilizer, CE/Uv-Vis and AA.Setup lab with instructor, made solutions, check the student's notebooks and helped students complete lab experiments. Specific examples unique to the different labs are spelled out below:Quantitative Analysis Taught basic laboratory techniques as scientific calculations, pipetting, titratations, pH testing and weighing. Verified students calculations, pH of prepared solutions and checked concentration of solutions by UV-Vis. Graded all laboratory notebooks to monitor for quality of work and completion of required tasks.Analytical Instrumentation Experiments were prepared and tested prior the students' labs on such instrumentation as UV-Vis, HPLC, GC, CE, AA, and also tested sample preparations techniques such as SPE.Taught and ran CE/UV-Vis laboratory experimentMolecular Biology Grew cell cultures, isolated various plasmids, PCR, prepared agar plates, liquid culture media and performed checks on student samples by gel electrophoresis and UV-Vis.; Performed analytical tests requested by customers.Checking for purity of samples using FTIR.Determining concentration for shelf life and to check compounds made by customers have the correct labeled drug concentration by HPLC and UV-Vis spectroscopy using known methods or methods that were researched and developed our lab.

Filled prescriptions, compounded creams and suspensions and data entry.Duties as inventory specialist includes controlling the pharmacy's inventory by maintaining adequate supply on high demand drugs, ordering filling supplies such as vials and bottles, controlling spending to outside warehouse to save the company money, making sure drugs were not expired and returning expired drugs to an outside warehouse in a timely manner.Duties as lead pharmacy technician included training employee, being a resource for insurance billing, pharmacy law and NC Medicaid laws, completing required paperwork and making weekly schedule.

Support research, actives and projects.Apply advanced level project methodology with limited guidance.Applied appropriate methods and techniques in performing experiments.Kept detailed records and documentation of laboratory actives, results and projects.Hands on use of the following laboratory instruments: HPLC, LC/MS, liquid handlers, UV-Vis spectroscopy, pipettes, and balances.

Master Of Science, Pharmaceutical Science

Focus on Biotechnology/Bioprocessing and Analytical Chemistry

Bachelor Of Science, Pharmaceutical Science

Education record

College transfer courses Focus on Medical Laboratory Technology MLT Courses taken: Clinical Chemistry, Microbiology, Urinalysis and Body.