Focus: Developing and executing functional genomics and deep mutational scanning screens for proprietary proteins. As part of this, led assay development for growth and flow cytometry assays, designed and performed screens, and established training and documentation frameworks for pooled screening within the company.

Focus: Deep Mutational Scanning, CRISPR, ORF, and Base Editing Screens, specializing in the design and interpretation of pooled mutagenesis screens for therapeutic targets. Collaborated and consulted on ~79 screening projects. Optimized screening strategy, troubleshooting, interpretation and quality controls. Initiated, designed and wrote a grant for experiments to pressure test new screening technologies. Led a monthly Screeners Meeting where collaborators could share their experiences with screening. Group: Genetic Perturbation Platform, Directed by David Root, PhD.

Focus: Identification of common ATP-competitive Inhibitor Resistance Sites in Kinases utilizing Deep Mutational Scanning pooled screening. Hypothesized, designed and tested comparative mutagenesis functional assays to identify drug resistance in cancer therapies for ultimate prevention. Supported multiple other Cancer Program projects as part of a highly collaborative team. Served as a committee member for the “Innovators in Cancer Research” seminar seriesGroup: Cancer Program, Group of Cory Johannessen, PhD.

Focus: Mechanisms of Fanconi Anemia Proteins in Crosslinked DNA Damage Repair. Purification, crystallization, biochemistry and interpretation of protein complexes of FancI and FancD2 in the presence of various types of DNA. Initiated work on Fanconi Anemia Mono-ubiquitination Complex from zebrafish and human constructs. Synthesized and purified covalently crosslinked DNA using click-chemistry for use in structural and biochemical experiments for Fanconi Anemia proteins and the Fan1 exonuclease. Executed skilled protein production from Bacteria, Insect, and Mammalian cell culturesGroup: Structural Biology Department, Lab of Nikola Pavletich, PhD

Focus: Biochemistry of the DNA Replication and Nutritional Stress Response Protein ObgE. Purified untagged proteins, undertook GTPase assays, (p)ppGpp binding assays, and cell death assays with DNA damaging agents. Additionally used quantitative microscopy to follow DNA replication in wild type and mutant cells.Group: Biophysics and Biology Departments, Lab of Susan Lovett, PhD.

Focus: Sequencing BRCA2 and FANCD2 Mutations in Fanconi Anemia Patients.Group: Molecular Diagnostics Laboratory, Lab of Edward Fox, PhD.

Focus: Copper-Pyrazine Compounds and Mn-12 thin film synthesis.Group: Chemistry Department, Lab of Sarah Stoll, PhD