Topic- Studying molecular mechanisms for Cry protein resistance in soybean looper (Chrysodeixis includens) and sunflower looper (Rachiplusia ni). - Currently working on NGS data from soybean looper including functional annotation of genome and RNAseq data.

- Conducted research on the resistance mechanisms of Two-Spotted Spider Mite (TSSM) to acaricides, focusing on abamectin resistance.- Employed various assays, including fluorescent probe competitions and UPLC analyses, to study the metabolic capacity of CYP392A11 and CYP392A12.- Performed transcriptomic profiling of resistant TSSM strains, identifying significant overexpression of CYP392 family P450s.- Found target site mutations in glutamate-gated chloride channels (GluCl), contributing to reduced acaricide efficacy.

- Contributed to the development of a comprehensive Bio-oxidation tool-library, encompassing the entire process from Plasmid construction to Biocatalysis, resulting in improved efficiency and efficacy of bioprocesses.- Assisted in the construction of CRISPR-plasmids to facilitate genome-integration, enabling precise genetic modifications.- Supported projects using molecular biological, protein biochemical, bioanalytical, and biocatalytic techniques, contributing to the success of ongoing research.

- Utilized next-generation sequencing (NGS) to accurately identify and analyze 140 seaweed samples collected from coastal regions in Europe and Africa, contributing to the understanding of regional biodiversity and ecological patterns.- Achieved a 95% success rate in optimizing, developing, and executing DNA extraction experiments, ensuring the availability of high-quality DNA samples for downstream molecular analyses.- Proficiently performed a range of molecular biology techniques including DNA extraction, Gel Electrophoresis, Gradient PCR, and Nested PCR, ensuring accurate amplification and detection of target sequences.- Employed the Primer Indexing Method to efficiently prepare samples for next-generation sequencing, facilitating high-throughput sequencing and enabling comprehensive analysis of genetic diversity within the seaweed samples.- Demonstrated responsibility for the end-to-end process of experiment planning, execution, and evaluation, ensuring adherence to established protocols and meticulous documentation of results.

- Conducted a comprehensive study of the eukaryotic fumarase gene's evolutionary origin from class I and class II prokaryotic fumarase, leading to the discovery of its evolutionary lineage.- Developed a Linux-based bioinformatics data analysis pipeline, resulting in an optimized workflow for analyzing genomic data sets, reducing processing time by 30%.- Constructed and interpreted a phylogenetic tree using relevant genes, concluding that the fumarase gene evolved from class II with 85% Bootstrap value, providing insight into the evolution of the gene and its potential biological functions.

- Conducted a research project investigating the effect of mutations in the acetohydroxyacid synthase enzyme and studying their impact on substrate specificity.- Executed successful transformation and expression of genes into E. coli. Further, analysed the expressed proteins by acetohydroxyacid synthase enzyme by HPLC.- Analyzed HPLC data using Xcalibur software, identifying key patterns and trends which were utilized to characterize the products of protein assays with a 90% accuracy rate.

- Identified over 100 different bacteria types associated with sugarcane, resulting in a higher understanding of microbial activity within the crop. - Developed a comprehensive system for collecting, managing and processing sugarcane samples.- Designed and conducted an experiment to isolate, cultivate and identify bacteria using MALDI-TOF-MS.- Conducted phosphate and potassium solubilization assays to assess bacterial activity and metabolic reactions, providing valuable insights into bacterial behaviour and leading to the development of more effective crop management strategies.

• Collection and statistical data analysis on microbial strains as biofertilizers in the market.• Identification of efficient strains for industrial production