Nikita Kamatkar Email & Phone Number

Monheim, North Rhine-Westphalia, Germany

Topic- Studying molecular mechanisms for Cry protein resistance in soybean looper (Chrysodeixis includens) and sunflower looper (Rachiplusia ni). - Currently working on NGS data from soybean looper including functional annotation of genome and RNAseq data.

Monheim, North Rhine-Westphalia, Germany

- Conducted research on the resistance mechanisms of Two-Spotted Spider Mite (TSSM) to acaricides, focusing on abamectin resistance.- Employed various assays, including fluorescent probe competitions and UPLC analyses, to study the metabolic capacity of CYP392A11 and CYP392A12.- Performed transcriptomic profiling of resistant TSSM strains, identifying significant overexpression of CYP392 family P450s.- Found target site mutations in glutamate-gated chloride channels (GluCl), contributing to reduced acaricide efficacy.

Leverkusen, North Rhine-Westphalia, Germany

- Contributed to the development of a comprehensive Bio-oxidation tool-library, encompassing the entire process from Plasmid construction to Biocatalysis, resulting in improved efficiency and efficacy of bioprocesses.- Assisted in the construction of CRISPR-plasmids to facilitate genome-integration, enabling precise genetic modifications.- Supported projects using molecular biological, protein biochemical, bioanalytical, and biocatalytic techniques, contributing to the success of ongoing research.

Kiel, Schleswig-Holstein, Germany

- Utilized next-generation sequencing (NGS) to accurately identify and analyze 140 seaweed samples collected from coastal regions in Europe and Africa, contributing to the understanding of regional biodiversity and ecological patterns.- Achieved a 95% success rate in optimizing, developing, and executing DNA extraction experiments, ensuring the availability of high-quality DNA samples for downstream molecular analyses.- Proficiently performed a range of molecular biology techniques including DNA extraction, Gel Electrophoresis, Gradient PCR, and Nested PCR, ensuring accurate amplification and detection of target sequences.- Employed the Primer Indexing Method to efficiently prepare samples for next-generation sequencing, facilitating high-throughput sequencing and enabling comprehensive analysis of genetic diversity within the seaweed samples.- Demonstrated responsibility for the end-to-end process of experiment planning, execution, and evaluation, ensuring adherence to established protocols and meticulous documentation of results.

Kiel, Schleswig-Holstein, Germany

- Conducted a comprehensive study of the eukaryotic fumarase gene's evolutionary origin from class I and class II prokaryotic fumarase, leading to the discovery of its evolutionary lineage.- Developed a Linux-based bioinformatics data analysis pipeline, resulting in an optimized workflow for analyzing genomic data sets, reducing processing time by 30%.- Constructed and interpreted a phylogenetic tree using relevant genes, concluding that the fumarase gene evolved from class II with 85% Bootstrap value, providing insight into the evolution of the gene and its potential biological functions.

Kiel, Schleswig-Holstein, Germany

- Conducted a research project investigating the effect of mutations in the acetohydroxyacid synthase enzyme and studying their impact on substrate specificity.- Executed successful transformation and expression of genes into E. coli. Further, analysed the expressed proteins by acetohydroxyacid synthase enzyme by HPLC.- Analyzed HPLC data using Xcalibur software, identifying key patterns and trends which were utilized to characterize the products of protein assays with a 90% accuracy rate.

Pune, Maharashtra

- Identified over 100 different bacteria types associated with sugarcane, resulting in a higher understanding of microbial activity within the crop. - Developed a comprehensive system for collecting, managing and processing sugarcane samples.- Designed and conducted an experiment to isolate, cultivate and identify bacteria using MALDI-TOF-MS.- Conducted phosphate and potassium solubilization assays to assess bacterial activity and metabolic reactions, providing valuable insights into bacterial behaviour and leading to the development of more effective crop management strategies.

Pune, Maharashtra

• Collection and statistical data analysis on microbial strains as biofertilizers in the market.• Identification of efficient strains for industrial production

Master'S Degree, Molecular Biology And Evolution

Phd Student, Molecular Biology

PhD at Bayer AG in collaboration with University of Kiel- TransEvo program.