Nikita Savelyev Email & Phone Number

California, United States

Newark, California, United States

▲ Played a key role in transferring a part of the products from the AZ facility to CA.▲ Development and manufacturing of custom products according to ISO 9001.▲ R&D of potential products with the goal of optimizing labor and cost.▲ ADC linkers testing, Antibody-drug conjugation (small molecules, oligonucleotides, peptides) and AKTA FPLC purification (SEC, IEX, HIC), UF/DF, PAGE, Western Blotting, ELISA, UV-Vis, IHC, microscopy.

Scottsdale, Arizona, United States

▲ QC of products. ▲ Preparation for the transfer of part of the products from the AZ facility to CA after the acquisition of Click Chemistry Tools by Vector Laboratories.▲ Purification of products on prep-HPLC, analysis on HPLC and LC/MS.

Phoenix, Arizona Area

▲ R&D of new products from early concept to manufacturing including planning, experimental work, optimization, troubleshooting, scaling up, creating SOP and BR, and finally, manufacturing and QC. ▲ Technical support for clients regarding biology.▲ Protein enrichment, AKTA FPLC protein purification, Western Blotting, ELISA, solid phase peptide synthesis (SPPS), HPLC, prep-HPLC, UV-Vis, LC/MS.

Moscow, Russian Federation

▲ Managed 2 research projects in telomerase biology from the very beginning. Designed experiments, studied new methodologies, analyzed scientific literature and experimental data, presented results at conferences and seminars.▲ Performed DNA/RNA/protein manipulations, lentivirus production, transfections, molecular cloning, blotting, RT-qPCR, metaphase FISH, immunoFISH, immunocytochemistry (ICC), In Vitro transcription, cDNA synthesis, telomerase activity analysis (direct and TRAP), telomere length analysis, CRISPR/Cas9 gene editing, human cell lines engineering using lentivirus and CRISPR/Cas9, cell maintenance. ▲ Optimized Southern and Northern blotting methodologies, telomerase assembly analysis protocol, immunoprecipitation (IP) of H/ACA RNPs. Created non-PARylated forms of GAR1 and DKC1 (Dyskerin) proteins usind site-directed mutagenesis (PCR). Performed in Vitro PARylation assay.▲ Worked with siRNA-ligand conjugates to check the possibility of address delivery to cancer cells.▲ Mentored and trained students and junior colleagues; performed group seminars and workshops for undergraduate students

Moscow, Russian Federation

▲ Learned and developed basic methodologies, that became critical for research projects. Received training in working with radioactive compounds.▲ Performed DNA/RNA/protein manipulations. In Vitro transcription, cDNA synthesis, PCR, Cloning, telomerase activity analysis (direct and TRAP), telomere length analysis.▲ Cloned plasmid constructs encoding PARP1-BFP fusion protein. Created stable cell lines using non-specific recombination and G418 selection.▲ Received training in working with radioactive compounds using all safety measures