https://circuitbiotech.com/

Research and Development at Diabest Co. Drug development based on medicinal plants. The research includes characterization of active ingredients, developing methods of testing drug efficacy, working with cell cultures, RT-PCR and mice preclinical development. Some of the work includes writing reports, training, teaching and leading the project.

• Colorectal cancer and the Wnt signal: Research aimed to Identify new Wnt signal components by using a new genetic functional screen. I identified the enzyme carboxypeptidase E (CPE) and its splice variant, N-CPE, as novel regulators of the Wnt pathway and published one article in Oncogene.• Mass Spectrometry – Immunoprecipitation of complexes for identification of new interaction with CPE in mammalian cells. Identify two protein interactions that are now under investigation. • Gene therapy: Research aimed to find new genetic manipulations against cancer cells and tumors based on tumor specific gene expression (working with "Nude" mice). The research is in its final stage, the results will be published soon.• Drugs treatment: Researched the ability of several drugs to downregulate the Wnt signal. Min-/+ mice were Treated with drugs and their small and large intestine were examined for polypes. Among other aspects I examined the differential gene expression between treated and untreated mice by Microarray chip. • APC mutation polymorphism: Polyps from colon cancer patiens were examined for APC allele mutations polymorphism. • Aminoglycosides derivates: Examining the half-life and the treatment of new Aminoglycoside derivates on C57BL/6 and Min-/+ mice colorectal cancer.• Orientations: Experience with "nude" mice and C57BL/6 mice surgery and maintenance, mice injection treatment, CO-Immunoprecipitation, Western blot, PCR, Luciferase assay, tissue culture, siRNA, Immunofluorescence and Immunohistochemistry staining, Mass Spectrometry, mutagenesis, establish stable cell line, molecular methods, vectors cloning and RNA extraction from tissue for microarray chip. Extensive collaboration with laboratories in Israel and around the world.

• 2010–2014. Team leader, project management and training of graduate and master students - training includes management of research, guidance and teaching methods.• 2008–2014. Participation in writing articles, reports and submitting grants.

• Pharmaceutical products biochemistry and sol-gel chromatography: Focus on the development of immunochemical methods for monitoring environmental pollutants and drug residues in food, environmental and biological samples. Development of ELISA for the quantification and detection of NSAIDs combined with sol-gel chromatography (IAP) to clean and concentrate biological samples such as human serum, milk, water and sand. Published three articles in that manner.• PCBs ELISA combined with sol-gel chromatography: Development of an immunoassay and a sol-gel-based immunoaffinity cleanup method for coplanar polychlorinated biphenyls from soil and sediment samples. • Orientations: Extensive work with sol-gel-based immunoaffinity purification (IAP) chromatography, LC/MS/MS, SPE columns. Development polyclonal antibodies in rabbits aimed against NSAIDs drugs (Indomethacin and Atenolol) and antibodies characterization. Development of specific ELISA for the detection of substances in biological samples.