Niraj Patel, Ms, Phd Email & Phone Number

Boston, MA, US

Quebec City, Quebec, Canada

• Doctoral Project: Innovative Brain Disease Modeling using Human-induced pluripotent Stem Cells (iPSCs) Derived Cortical Neural Cultures and Multimodal Digital Holographic Microscopy with a Spotlight on NMDA Receptors.
• Utilized a multimodal digital holographic microscope (M-DHM), a non-invasive and high-resolution live cell imaging technique, to assess NMDA receptor activity during neurodifferentiation.
• Developed and optimized a standardized protocol for differentiating human induced pluripotent stem cells (iPSCs) into mixed cortical neuronal culture.
• Characterized the iPSC-derived mixed cortical neurons culture using fluorescence-activated cell sorting (FACS), Immunocytochemistry (ICC), Western blot (WB), quantitative-PCR, and Whole patch-clamp techniques to ensure.
• Demonstrated and established the transmembrane water fluxes (TWFs) in iPSCs-neurons and the foundation for a label-free imaging assay using iPSC-derived human neurons in vitro, with potential applications in.

Boston, Massachusetts, United States

Project: In Vitro and In Vivo Studies on Neural Circuits and Microglia: Unraveling the Role of Reelin-APOE Pathways in Neurodegenerative Diseases, with a Focus on Alzheimer's Using Rodent and Human Models.Leading in vitro and in vivo studies on Alzheimer’s disease and develope therapeutic interventions using human iPSCs and rodent models, contributing to.

Montreal, Canada Area

• Worked on a project entitled; To investigate the treatment of degenerative macular disease by using stem cell therapy.
• Generation, development and maintenance of human-induced pluripotent stem cells (iPSCs) In-vitro.
• Differentiation of iPSCs cells into Rod and Cone cells in In-vitro 2D culture for Pharmacological tests.
• Maintained various cell lines (ESCs, feeder cells, NIH3T3 and H293T) to conduct experiments.
• Performed reverse transcription-polymerase chain… Show more Worked on a project entitled; To investigate the treatment of degenerative macular disease by using stem cell therapy.
• Performed reverse transcription-polymerase chain reaction (RT-PCR) for RNA analysis.

Bangalore

• Project-1; Investigating the mechanisms for early diagnosis and treatment of Alzheimer’s disease using induced pluripotent stem cells (iPSCs) derived neurons.
• I developed in-vitro cortical neuron culture from human-induced pluripotent stem cells, which were derived from blood cells.
• Standardise in-vitro AD model for pharmacological screening and to understand the mechanisms of beta-amyloid plaques accumulation in sporadic AD.Project-2; Studying the role of… Show more Project-1; Investigating the.
• Standardise in-vitro AD model for pharmacological screening and to understand the mechanisms of beta-amyloid plaques accumulation in sporadic AD.Project-2; Studying the role of SCL/TAL1 interrupting locus (STIL) in.
• The project involves extensive work using a variety of approaches such as electroporation (NEPAGENE) to transfect human STIL gene to in vitro cultured cortical embryonic neurons and in vivo embryonic transfection.
• This study showed an altered neuronal morphology following transfection suggestive of morphological abnormalities as observed in MCPH patients. Show less

Bengaluru, Karnataka

• I gave Neurobiology practical training to 40+ undergraduate (UG) and Post-graduate (PG) students. The training was on;
• Mice handling, breeding and maintenance.
• Mice pub embryo isolation at a different time point.
• Maintenance and storage of stem cells.
• Cortical neuron differentiation and maintaining cell cultures.
• Immunocytochemistry and microscopy imaging and analysis.

Trivandrum, Kerala, India

• Project-1; The effects of maternal sleep deprivation on cognition in the offspring in an animal model,Project -2; The impact of acute maternal total sleep deprivation (TSD) at the third trimester of pregnancy in the.
• I performed stereotactic brain surgery on 100+ Wister rat to implant the electrode to study brain rhythm.
• Record Ultrasonic Vocalization (USVs) from rat pups and analyzed them with Avisoft and SAS-Lab.
• Performed EEG, EMG and EOG… Show more Project-1; The effects of maternal sleep deprivation on cognition in the offspring in an animal model,Project -2; The impact of acute maternal total sleep deprivation (TSD) at the.
• Performed EEG, EMG and EOG recordings and analysis by BioPac system (model 150) software and Data Sciences International (DSI) telemetric system.
• Cognitive behavior analyzed using the Any-Maze system, plus maze and open field test for anxiety.

Ahmedabad, Gujarat, India

• Support R&D and product Supply and assist in project/technology/method transfer in the QA/QC department.
• Review & approve batch records, documentation for records, product validation and stability testing at different time points and conditions using multiple laboratory tools.
• R&D supports the compliant and timely closure of all project work requiring Quality Assurance approval.
• Document review & approval, such as method validations, manufacturing records and… Show more
• Document review & approval, such as method validations, manufacturing records and Standard Operating procedures (SOPs) development.
• Maintenance of material safety data sheet (MSDS) and product data sheet (PDS) information for the company products and prepare technical reports.

Hyderabad, Telangana, India

• Project-1;Inflammatory study on Sprague Dawley (SD) rats; an arthritis model for drug development and screening.
• Regularly exercises technical discretion in design, execution and interpretation of experiments that contribute to project goals.
• Involved in teamwork, provide guidance to less senior personnel and providing solutions to problems associated with specific projects.
• Prepares technical reports, summaries, protocols and quantitative analyses and discusses… Show more Project-1;Inflammatory study on Sprague Dawley (SD) rats; an arthritis model for drug development and screening.
• Prepares technical reports, summaries, protocols and quantitative analyses and discusses the outcomes with supervisors and project team members. Show less

Hyderabad, Telangana, India

• Make documents to collect the raw material form production department to perform tests,
• Calibration of wet analysis instrument (KF titration, LOD, Sulphated ash etc.)
• High-performance liquid chromatography (HPLC) analysis,
• Gas Chromatography (GC) analysis,
• Preparation of datasheets and so on.

Hyderabad Area, India

• A High School Biology Teacher who understands the very best ways to get students interested in biology.
• Develop lesson plans and themes in line with curriculum objectives specific to teaching biology subjects.
• Instruct students through lectures, discussions, and demonstrations.
• Prepare, administer, and grade tests and assignments to evaluate student progress.

Rewa Area, India

• Responsible for handling questions, concerns and special requests from clients.
• Demonstrate professionalism at all times; on the phone, through email, and during meetings.
• Maintenance of pharmacy stock, Handling customer prescription and day-to-day operations of retail sales.
• Maintain a clean and organized workplace.
• Documentation of operation performed on a lot and computer data entry.

Doctor Of Philosophy - Phd, Neurosciences

PhD project: Exploring with multimodal-digital holographic microscopy the homeostatic plasticity during the neurodifferentiation of iPSCs.

M.Sc., Neuroscience

Activities and Societies: Awarded the best in the Group poster presentation 2009 on Science day, Organized by DST and Jiwaji University.

B.Sc., Biotechnology

Activities and Societies: Workshop on Recombinant DNA technology, Seminar on recent advances in biotechnology application, Cancer.