Nithya Thambi Email & Phone Number

West Point, Pa

West Point, Pa

Princeton, Nj

- Preclinical studies to evaluate the efficacy of novel genetically Listeria Monocytogenes (Lm) based immunotherapies for various cancer indications- Identify factors in the tumor microenvironment that contribute to the mechanism of action of the Lm therapies using various microscopy techniques to visualize immune cell distribution and phenotyping using multicolor flow cytometry including data analysis- Experienced in advanced immunology techniques including ELISA, cytokine detection and immune cell isolation from whole blood or tissues- Evaluate combination therapies that can be used in conjunction with the Lm-based immunotherapies- Responsible for supporting new and ongoing research collaborations- Publish abstracts, manuscripts and poster presentations

Tarrytown, Ny

- Tumor biology research focused on intracellular trafficking and processing of monoclonal antibodiesand Antibody-Drug Conjugates (ADC)- Validated potential drug targets by establishing complex in vitro cell systems and high throughput assay development including demonstrating in vitro efficacy of ADCs, developing functional and biochemical assays, live cell real-time monitoring assays, high content imaging utilizing automated high-throughput microscopy platforms and image analysis software (MetaXpress)- Characterized Patient-Derived-Xenografts and breast cancer patient tumor samples- Expert in culturing primary human endothelial cells and tumor cell lines, producing stable lines using plasmid transfections, 3D assays (spheroid, tube formation and fibrin bead assay), calorimetric and fluorescence based assays, trafficking assays, quantitative Flow Cytometry (Fortessa LSR and Accuri) to assess cell surface, cytoplasmic proteins and testing panel of biomarkers- Proficient in Western Blotting to visualize protein degradation & signaling pathways, immunostaining and antibody screening in multi-well formats, confocal microscopy, TAQMAN analysis, animal necropsies and RNA isolation- Published in peer-reviewed journals and poster presentations. Mentored, trained and managed graduate students and interns.

Philadelphia, Pa

- Developed a biomimetic, micro-scale drug delivery system using flagella-aided active magnetic propulsion system- Performed active flagella isolation from Salmonella bacteria, prepared buffers and media for bacteria culture and cell assays- Designed a propulsion system powered by magnetic fields, generated by a set of electromagnetic coils in a Helmholtz configuration- COMSOL and AutoCAD used to design working prototype- Controlled motion of the device with flagella filaments established using a LabView interface- Performed image-based analysis using MATLAB and SEM.

West Point, Pa

- Performed, designed cell based and in vivo drug concentration varied assays to determine intra-cellular trafficking of siRNA, targeted towards an effective cure for cancer- Performed in vitro multi-well plate-based bioassays. Conducted RNA purification, transformation and transfection- Validated reagents, prepared media, buffers and developed protocols for fluorescent microscopy to analyze siRNA delivery platforms along with statistical analysis of data using PRISM- Performed image-based analysis using the InCell softwares and the Tecan Safire

Malvern, Pa

- Developed MMI (Man Machine Interface) graphics from P/I diagrams- Associated each interface block with tag database for operator consoles utilizing Allen-Bradley RS View 32 and Wonderware development software- Conducted shop inspections to insure project functionality

Master'S Degree, Biomedical Engineering

Bachelor Of Science - Bs, Bioengineering And Biomedical Engineering