Nitin Kumar, Phd Email & Phone Number

Bengaluru, Karnataka, India

1. Team lead for early-stage fermentation process development and late-stage scale-up.2. Actinomycetes and Gram-Negative microbe based immunosuppressant and antibiotic process development and cost improvement at plant scale3. Genome-Scale Metabolic Modelling for Secondary metabolites and recombinant peptides flux analysis4. Strain improvement by classical as well as metabolic modelling based flux analysis approaches5. Interdepartmental coordination with Analytical R&D, MSAT, QA, Regulatory Affairs and Supply Chain team for regulated market targeted process development6. Portfolio Selection, Feasibility Analysis and Cost Estimation for potential new molecules

Hyderabad Area, India

1. Active in commissioning and qualification of the Single Use-Hybrid cGMP facility of 4KL scale for regulated markets2. GE Single-Use Bioreactors (SUBs) (200L, 500L and 2KL) Installation and Qualification3. GE SUBs (200L, 500L and 2KL) Engineering Characterization by KLa and P/V methods4. GxP, URS, GAP, GAMP and Risk assessment of the Equipment with associated process steps followed by IQ/OQ qualifications5. MCB and WCB establishment6. SOP preparation for GE SUBs7. Point of contact for Up-Stream Scale-up and Technology transfer8 Part of the interdepartmental team for Quality Management System (QMS) establishment

Hyderabad Area, India

Team Lead for Insulin Analogues' clone and process development

Guwahati Area, India

1. Cloning human Interferon Gamma in Bacillus subtilis & E. coli, Pichia pastoris & Kluyveromyces lactis Generally Regarded As Safe hosts2. Design of Experiments (DoE) statistical approach for culture medium optimization & Bioreactor Critical Process Parameter Optimization for recombinant therapeutic protein human Interferon Gamma production and process development3. Machine Learning: Artificial Neural Network Artificial Intelligence (ANN-AI) based culture medium optimization and Bioreactor Process Parameter Optimization4. Application of Genetic Algorithms and Simulated Annealing Algorithms for Artificial Neural Network Optimization and Critical Process Parameter identification to establish specified parameter range5. Metabolic Modelling of the cultivation process for human interferon gamma production using flux balance analysis (FBA), Elementary Mode Analysis (EMA) approach in MATLAB following Non-Linear Optimization Algorithms & stoichiometric analysis of the metabolic network for Upstream Process Development. Identification of critical media attributes & bioreactor process parameters for interferon gamma production using stoichiometric modelling. Oxygen requirement calculation for the bioreactor using stoichiometric modelling & KLa calculations6. Ordinary Differential Equation (ODE) based kinetic modelling & Critical Parameter Estimation for recombinant human Interferon Gamma Production using MATLAB based Numerical Mathematics programming7. Development of Fed-Batch HIgh Cell Density Cultivation (HCDC) Production Process and optimum feeding profile based upon stoichiometric yield analysis & Ordinary Differential Equation based modelling of the batch process.9. Scale-up of the process up to 7L working volume capacity in a 10L bioreactor using constant KLa method.8. Purification of human Interferon Gamma from the extracellular fermentation broth & the intracellular inclusion bodies using HIS-Tag, Ion Metal Affinity Chromatography (IMAC) in GE AKTA set-up.

Hyderabad Area, India

Lisbon Area, Portugal

1. Fed Batch Process High Cell Density Cultivation of Bacillus subtilis for Human Interferon Gamma production. 2. Genetic engineering of E. coli for higher Human Interferon Gamma production and metabolic modelling

Meerut Area, India

1. Isolation and characterization of Trichoderma and Fusarium isolates.2. Cultivation and interaction of Trichoderma harzianum and Fusarium lycopersisi.3. mRNA isolation and RT PCR for gene expression profiling.

Jalandhar Area, India

Taught masters students: Bacteriology, Fermentation Technology, Bio process Engineering and Immunoprophylexes.

Jammu And Kashmir, India

1. Large Scale (500L) Fermentation process development for nutraceutical Omega 3 Fatty Acid DHA production from a marine algae.2. Fed-Batch Fermentation process development (10L) for enhanced DHA production.3. Scale-Up of the Fermentation Process from 5L to 50L and 500L by Constant KLa method using empirical correlations b/w gassed and ungassed power consumption, superficial air velocity and KLa along with corrections for reactor geometries and impeller geometry.4. Tubular bowl centrifugation for cell separation, supercritical fluid extraction and solvent extraction for oil recovery from cells.5. Winterization process for Docosahexaenoic acid purification from saturated fatty acids.6. Operation and handling of the fermentation pilot plant facilities like Air compressors, Air dryers, Boilers, Scigenic Reactors upto 500L etc.

Doctor Of Philosophy (Ph.D.), Fermentation Tech., Bioprocess Development, Metabolic Engg., 9.1

Erasmus Mundus Experts Phd Exchange, Biochemical Engineering

Master Of Technology (M.Tech.), Industrial Biotechnology (Biochemical Engineering), 9.0, Gold Medal Deptt. Of Chemical Engineering

Bachelor Of Technology (B.Tech.), Biotechnology, 8.3