Nazareno Menegatti Email & Phone Number

Italy

Via Meucci 3 - 20091 Bresso (Milano, Italia)

Via Meucci 3 - 20091 Bresso (Milano, Italia)

Via Meucci 3 - 20091 Bresso (Milano, Italia)

Compound Management at AXXAM involves automation, handling, procurement, storage and organization of compound libraries. AXXAM has the capacity to store and handle compound libraries either in a Brooks Universal Labstore 600 or in a REMP facility. Compound arrival, registration and handling is performed according to internal Standard Operating Procedures. Compound management is able to provide several services, including plate copying, dilution preparation, assay-ready plate, cherry-picking of… Show more Compound Management at AXXAM involves automation, handling, procurement, storage and organization of compound libraries. AXXAM has the capacity to store and handle compound libraries either in a Brooks Universal Labstore 600 or in a REMP facility. Compound arrival, registration and handling is performed according to internal Standard Operating Procedures. Compound management is able to provide several services, including plate copying, dilution preparation, assay-ready plate, cherry-picking of hit and custom libraries. All these activities are supported by the full integration of the instrumentation with AXXAM’s screening database (SB3). Show less

Via Meucci 3, Bresso (Milano, Italia)

Axxam provide access to three fully automated state-of-the-art screening stations designed to run biochemical and cell-based assays in 384 well format, using a variety of detection technologies including luminescence, fluorescence, absorbance, HCS and radiometric readouts. Compound management activities are carried out with Hamilton StarLET and CyBi-Felix liquid handlers, and the automatic compound storage is done using a Brooks Universal Labstore UL-600 (plate-based) and Hamilton Verso Q50… Show more Axxam provide access to three fully automated state-of-the-art screening stations designed to run biochemical and cell-based assays in 384 well format, using a variety of detection technologies including luminescence, fluorescence, absorbance, HCS and radiometric readouts. Compound management activities are carried out with Hamilton StarLET and CyBi-Felix liquid handlers, and the automatic compound storage is done using a Brooks Universal Labstore UL-600 (plate-based) and Hamilton Verso Q50 (vial-based). As automation lab scientist, I was in charge of managing and programming of these instruments with the aim of finding the proper way to carry out day-by-day activities. Show less

Via Meucci 3 - 20091 Bresso (Milano, Italia)

Axxam offers access to a next-generation small molecule collection for hit finding by high-throughput screening, of around 325,000 drug-like and lead-like compounds. It consists of four independent sets accessible separately or in combinations according to project needs.As computational chemist, I am part of the team that selected and designed the whole library of compounds, with the goal of ensure that the identified screening hits could represent promising and favorable starting points… Show more Axxam offers access to a next-generation small molecule collection for hit finding by high-throughput screening, of around 325,000 drug-like and lead-like compounds. It consists of four independent sets accessible separately or in combinations according to project needs.As computational chemist, I am part of the team that selected and designed the whole library of compounds, with the goal of ensure that the identified screening hits could represent promising and favorable starting points for further optimization: main drivers are high quality of properties, purity of stocks and strong chemical novelty and diversity.In addiction I usually take care of clustering, chemical scaffold analyses, preliminary SAR reports. Show less

Via Meucci 3 - 20091 Bresso (Milano, Italia)

Description:- devise, construction and use of medicinal chemistry filters which eliminate compounds with problematic features- HTS possible false negative recovery- cluster and scaffold analysis- preliminary SAR, SAR analysis and hit prioritization- in silico ADMET prediction- pharma database searching- chemical libraries managing- High-throughput screening data managing- Library design

Via Olgettina, 58 Milano (Mi)

Thesis title: "Innovative methods for virtual screening of molecular libraries targeted to drug design of HDAC7 protein inhibitors".Description: deepening of the methodologies workflow that allows the construction of a library of commercial compounds with the aim to search for possible HDAC7 inhibitors. The workflow includes: - database search using Accelrys Pipeline Pilot protocols;- construction and design of structural chemical queries using Accelrys' software Accord Draw… Show more Thesis title: "Innovative methods for virtual screening of molecular libraries targeted to drug design of HDAC7 protein inhibitors".Description: deepening of the methodologies workflow that allows the construction of a library of commercial compounds with the aim to search for possible HDAC7 inhibitors. The workflow includes: - database search using Accelrys Pipeline Pilot protocols;- construction and design of structural chemical queries using Accelrys' software Accord Draw with a ligand-based approach;- construction and use of medicinal chemistry filters (based on Lipinski rule of five, known frequent hitters and reactive chemical moieties) which allow the selection of a particular group of compounds inside the molecular library;- definition of pharmacophoric models using a structure-based approach and the Accelrys' software Discovery Studio;- molecular docking simulation using the Discovery Studio and CDOCKER (CHARMm-based) algorithm;- application of multi-objective optimization algorithms.Other projects: - SAR analysis of TRPM8 ligands;- molecular docking simulations using VEGA ZZ software;- SMARTS (SMiles ARbitrary Target Specification) strings writing. Show less

Via Lurani, 12 Bresso (Mi) 20091

Perform molecular docking simulation tests on several ligand-protein complexes using two different softwares (DELOS and AutoDock); analysis and comparison of results for the validation of the DELOS' scoring function.

Via Lurani, 12 Bresso (Mi) 20091

Design and build 20 amino acid variants of a bridge linking two subunits of an enzyme; test the binding activity of the 20 different proteins obtained in this way with the natural ligand using molecular docking simulations with software DELOS.

Msc Degree, Bioinformatics, 110/110 Cum Laude

Biomathematics, Bioinformatics, and Computational Biology

Bsc Degree, Biotechnology (Molecular Curriculum), 104/110

Diploma, Scientific Lyceum, 100/100