I am a Biochemistry PhD student at the Kastrup lab (Medical College of Wisconsin & Versiti Blood Research Institute, formerly the University of British Columbia) with a passion for translational blood research. My interests lie with transfusion medicine, RNA therapeutics, and blood cell bioengineering. We utilize lipid nanoparticles (LNPs) to deliver si/mRNA delivery vessel into blood cells, such as platelets. This technology can potentially modulate cellular function, with implications ranging from treating deadly bleeding disorders and degenerative diseases to mitigating lethal hemorrhage.__________________________________________________________________________________________________________________SKILLS:Practical:-Bacterial and eukaryotic cell culture-Plasmid mutagenesis and cloning-DNA isolation and purification-mRNA synthesis, isolation, purification, and expression in cell lines (lipofectamine, LNPs)-LNP formulationAnalytical:-Protein analysis/quantification (BCA/Bradford assay, SDS-PAGE, western blot, ELISA)-DNA/RNA analysis/quantification (PCR, RT-qPCR, agarose gel electrophoresis)-Cell & tissue analysis (Flow cytometry, widefield fluorescence microscopy, confocal microscopy)Animal models:-Mouse (tail vein injection, anaesthesia & euthanasia, cardiac puncture, bone marrow collection)-Swine (handling)