Nicholas R. Email & Phone Number

San Francisco, CA, US

United States

La Jolla, California, United States

• Generated and analyzed pilot screening data for every new enzymatic target, compound library, and biochemical assay on-boarding for in-house one-bead one-compound DNA encoded library (OBOC DEL) screening system.
• Wrote pilot screen data analysis pipeline in JMP for new target onboarding and new library onboarding, and automated experimental process on-system
• Assisted in the generation of and testing of new optics designs for proprietary screening instrument and enabled new modalities of screening (including fluorescence polarization and FRET)
• Redesigned solid phase library suspension buffer formulation using a DOE approach to prevent catastrophic flocculation while maintaining assay compatibility, microfluidic droplet stability, and enabling screening of.
• Collaborated on NGS prep improvement project to improve fidelity for DNA encoded library decoding pipeline utilizing Illumina NextSeq 2000 instrument. Proposed and integrated UDG cleanup step to prevent amplicon.
• Designed and implemented Taqman qPCR assay for detecting control compounds in high throughput system and performed validation of screening strategies via ROC analysis.

San Diego, California, United States

• Conducted high throughput screening on proprietary droplet microfluidics based system
• Tested new microfluidics designs and participated in the design make test learn cycle for both positive pressureand positive displacement setups
• Integrated new hardware into proprietary screening platform under supervision from senior engineer, SystemIntegration Group Lead
• Collaborated on project to train a machine learning model to identify and count hit library beads in sortedmicrofluidic droplets to statistically account for false positives.

San Diego, California, United States

• Collaborated with my team to develop a high throughput computational pipeline to generate interfering peptides for Alzheimer’s Disease morphology tau protein aggregates.
• Leveraged trRosetta for structure prediction, FlexPepDock for binding energy prediction, and pyMOL for protein structure viewing and analysis
• Utilized high performance computing techniques to perform high throughput in-silico biology.

San Diego, California, United States

• Independently designed plate imaging and MATLAB image processing pipeline for automated bacterial colony counting
• Provided lab maintenance ranging from specialized media preparation to handling hazardous and biohazardous waste streams

Pasadena, California, United States

• Independently designed and constructed droplet velocity selection filter equipment for use under high vacuum
• Prepared bacterial samples for digital holographic microscopy (DHM).
• Produced sample chambers in a cleanroom environment for DHM
• Leveraged Fiji/imageJ to analyze DHM datasets and performed 3D cell tracking for motility studies

Bachelor'S Degree, Bioengineering: Biotechnology

Activities and Societies: Research, Sailing

Education record