Nicholas A. Email & Phone Number

Bucharest, Romania

I teach undergrad applied statistics in the Computer Science section of the Department of Cybernetics, Statistics and Informatics.

Bucharest, Romania

• Participate in the clinical evaluation and medical therapy management for patients with pituitary, thyroid, parathyroid, adrenal, gonadal and pancreatic disorders• Write history and physicals, orders, progress notes, discharge summaries for inpatients, and medical letters and prescriptions for outpatients• Provide initial evaluation of new patients and draft work-up and management plans• Attend morning turnovers, morning reports, grand rounds, and noon conferences• Rotated in Adult Endocrinology, Pediatric Endocrinology, Internal Medicine, General Peds and other medical inpatient departments, most commonly at Filantropia City Hospital and the National Institute of Endocrinology.

Galaţi, Romania

• Provided initial diagnosis and treatment for acute conditions in a busy Family Medicine practice.• Prescribed and adjusted therapy for returning patients with common conditions.• Offered primary prevention counseling and screening for common diseases in an adult population.• Initially working under a provisional license and under supervision; subsequently independently licensed as provider of Family Medicine services in Romania.

Boston, Massachusetts, United States

• Collaborated in development of RCTs evaluating effectiveness of healthcare interventions based on genomic information accrued by Million Veteran Program, world's largest biobank (MVP-ROAR, NCT04178122 and GenoVA, NCT04331535)• Abstracted measurable outcomes for data capture and retentions systems, based on the approved protocol• Provided refinements to study instruments, in oral communication, as well as in edits to regulatory submissions (IRB protocols, grant submissions, SOP manuals, and progress reports)• Guided clinical research coordinators towards a 90% reduction in errors during data collection• Queried largest medical data warehouse in US (VHA’s CDW) for observational and trial selection purposes• Prototyped and built data entry systems in UML, RedCAP, Qualtrics• Processed heterogenous data with SQL, R, SAS, Excel / Access / VBA, Powershell, PowerBI, yielding scientific charts and executive dashboards• Developed, refined and validated EHR phenotypes, using inpatient, pharmacy, and cancer registry data• Measured accuracy of automated phenotyping through chart reviews for hundreds of patients• Reconciled variant adjudications from public data sources (ClinVar) and proprietary (Invitae) datasets.

Boston, Massachusetts, United States

• Created standardized laboratory protocols and led their implementation by students and teaching fellows• Taught spectrophotometry, PAGE, immunoblot, ELISA, IF microscopy, analytical and preparative chromatography, as theory and in hands-on laboratory sessions• Discussed circular dichroism, mass spectrometry, cell sorting, using digital analysis of sample data• Introduced the students to the principles of advanced experimental data analysis (estimates of error)• Created visual and text instructional materials, including the equivalent of a new textbook• Managed teaching assistants’ schedules and budgets.

Boston, Massachusetts, United States

• Ascertained mechanisms by which testosterone and IGF-I alleviate glucocorticoid-induced muscle atrophy• Planned and executed evidence-building experiments, in collaboration with R1-afiliated senior scientists.• Wrote research plans, budget drafts, and progress reports, assessed by supervising committee.• Wrote protocols describing standardized and safe work with research animals (IACUC)• Measured relative importance of synergistic pathways, using dose-ranging, chemical inhibition, and genetic depletion (siRNA) approaches in immortalized cell lines, freshly isolated stem cells, primary tissue cultures, transgenic cells and animals• Employed preparative ultracentrifugation, immunofluorescence (including confocal), fluorescent and bioluminescent reporter assays, immunoblot, immunoprecipitation, quantitative RT-PCR• Automated large-scale experiments on Tecan Saphire, Applied Biosystems ABI, Nikon Eclipse platforms• Evaluated protein synthesis and degradation through enzymatic assays and vital radioactive tracer labeling• Assayed human and rodent specimens in accordance with Biosafety Level 2 rules• Automated histology image processing using Bash and Matlab scripts, on remote Linux-powered clusters• Wrote reproducible research reports, paper sections, and dissertation, in LaTeX / R / ggplot2 (available at https://github.com/nvalexander).

Boston, Massachusetts, United States

• Spearheaded the implementation of Canary, a Perl-based text mining application which applies a decision-tree paradigm to clinical narratives, with the goal of enabling detection of poorly documented drug side effects• Led the development and implementation of its user-friendly interface using C#, allowing end users with limited technical experience build a rulebook comprising regular expressions, sentences, and anaphoras• Introduced code versioning (Git) and issue tracking (Bitbucket) systems • Gave practical demonstrations to clinician groups, vastly broadening our tool’s user base.

Boston, Massachusetts, United States

• Developed a research project through literature review and preliminary data collection, successfully submitting it to the Qualifying Examination Committee of the Department of Physiology and Biophysics• Probed macromolecular interactions, including between translation initiation factors eIF1A and eIF5B, apolipoprotein E and phospholipids, or surfactant protein A and mannose-containing polysaccharides• Optimized sequence, overexpression and purification protocols for tens of recombinant proteins• Purified recombinant and endogenous biomolecules by affinity, ion exchange, size exclusion chromatography, on Bio-Rad BioLogic DuoFlow, and GE Healthcare AKTA systems• Assayed products, and characterized protein-protein interactions, using gel electrophoresis, size exclusion chromatography, structural NMR, fluorescence anisotropy, and turbidity assays• Evaluated molecular conformations with molecular dynamics in PHENIX / Python and Bash• Analyzed findings in Sigmaplot, Origin, and Graphpad• Led student journal clubs.

Princeton, New Jersey, United States

• Investigated neurophysiological phenomena that correlate with the speedy extraction of statistics from visual scenes that contain many similar objects• Collected anatomic and functional human brain images, amassing hundreds of hours as lead MRI operator• Assisted in electroencephalography, eye tracker, transcranial magnetic stimulation experiments• Recruited, consented, instructed, debriefed, and measured cognitive abilities of hundreds of subjects• Developed visual stimuli in Matlab, using real-time video toolkits• Processed fMRI and behavioral data using SPSS, Bash, AFNI, locally, and on the university HPC Unix cluster• Wrote standardized instructions, debriefing statements, and consent forms• Drafted standardized protocols for ethical and safe research with human subjects (IRB)• Managed laboratory shared resources, including experimental devices, computers, rooms.

New York, New York, United States

• Shadowed physicians and nurses during interviews and physical examinations, in various departments of St Luke & Roosevelt Hospitals, Beth Israel Medical Center, and Beth Israel Kings Highway Division• Provided continuous feedback to medical staff during their transition to a custom version of Centricity.

Bucharest, Romania

• Diagnosed patients, under supervision, in Internal Medicine and Surgery departments• Provided basic treatment under supervision and discussed management for more complicated cases

Doctor Of Philosophy - Phd, Molecular And Translational Medicine

Master Of Science - Ms, Statistics And Data Science

Doctor Of Medicine - Md, Medicine

Bachelor Of Science - Bs, Biophysics