DNA Technologies Core: PacBio specialist 3rd Generation Sequencing Platform (long-read):* PacBio Platform Lead Specialist* PacBio Revio (March 2023-present)* PacBio Sequel IIe (Dec 2020 - 2024)* PacBio Sequel II (Sept 2019-2023)* PacBio Sequel (July 2016 - Sept 2019)* PacBio RS II (2012* PacBio RS (2011)* Manage and perform PacBio platform: workflow, projects, customer service, construct libraries, prepare libraries for sequencing, perform and deliver primary and secondary sequence data*Manage and operate sequencers* Provide customer service by email, phone, virtual meeting, and in-personGenome Mapping Platform* Bionano Saphyr * Lead Specialist* Managed and operated Saphyr* Managed projects, provided customer service, isolated ultra-high molecular weight gDNA, labeled/stained gDNA for optical mapping, and delivered dataNext Generation Sequencing (NGS) Platforms: * 3 Illumina GA IIx (Genome Analyzer) with 2 PE modules and 1 Cluster Station (2009)* Illumina HiSeq 2000* Illumina HiSeq 2500* Illumina HiSeq 3000* Lead Specialist * Managed projects, provided customer service, constructed libraries and prepared libraries for sequencing, operated the sequencers, and delivered sequence dataNext Generation (short-read) & Third Generation (long-read) Sequencing Library Preparations:* gDNA* Amplicons * total RNA* cDNA* mRNA* strand specific RNA* DSN treatment* Ribo-Zero rRNA* Apollo 324 Automated NGS Library Preparation SystemMicroarrays:* Illumina Whole-Genome Expression Beadchips for Gene Expression AnalysisEquipment Trainer**/User:* Agilent Bioanaylzer*** Diagenode Bioruptor UCD 2000*** Diagenode Bioruptor NGS*** Diagenode Megaruptor*** Diagenode Megaruptor 3*** Sage Blue Pippin HT & Blue Pippin*** Caliper GX Labchip* Agilent Femto Pulse* LightBenchLibrary Preparation Workshop Instructor:* RNA-seq* DNA-seq* PacBio

DNA and Diagnostic Testings for animals: Parentage and STRand Analyst/Technician▪ DNA Sequence Analyst: Analyzed electropherograms of feline and equine microsatellite markers produced on an ABI 3730xl 96-Capillary DNA Sequencer for parentage verification or trait diagnosis using proprietary VGL software (STRand Analysis Software) ▪ Genetic Analyst, Equine Coat Color Testing: Evaluated horse coat color phenotypes based on genotype; reviewed results and sent out final reports to clients ▪ Microsatellite-based Elk and Goat Parentage Analyst: Compared elk and goat offspring DNA profiles to those of the presumed parents. Analysis included determining the need for retesting of samples and possible use of additional DNA markers to confirm the exclusion of any non-qualifying parent(s) ▪ Lead Technician, Canine Parentage and Diagnostic Testing: Extracted DNA from canine blood, tissue, hair roots, and buccal swabs for PCR amplification ▪ Alternate Safety Coordinator, Veterinary Genetics Laboratory: Assisted with quarterly safety meetings, chemical inventory and disposal, and CUPA compliance ▪ Laboratory Maintenance and Quality Control: Archived canine samples according to tissue types; stored extracted DNA samples in an organized manner in freezers; proofread submission form entries for missing or inaccurate information; monitored and maintained a consistent supply ofreagents and consumables; identified testing failures and other problems and initiates troubleshooting steps to evaluate them