Olga Allen Email & Phone Number

Boston, Massachusetts, United States

Lead a team of scientists in the development and optimization of high-throughput screening assays to enable projects involved in gene therapy, microbiome health, vaccine adjuvants, and novel beauty ingredient discovery. Design customized integrated platforms (HRB), develop technical plans, and identify preferred vendors for hardware components and software packages, aligning with our scientific and technical requirements.Analyze large datasets and effectively communicate results to both internal and external teams.Develop and optimize liquid handler protocols for high-throughput assay platforms, leveraging expertise with Prime, Lynx, and Hamilton liquid handlers.Scale down cell-based assays to high throughput, executing compatible automated workflows that maintain sample and data integrity consistency within 96 and 384 well formats.Provide end-to-end guidance encompassing assay development, execution, analysis, and data reporting across various areas, including high-throughput ELISA, flow cytometry, functional screening, and protein characterization.Collaborate with in-house automation and data teams to seamlessly integrate high-throughput screening workflows into our internal systems.Design and troubleshoot laboratory automation systems to ensure the efficient and robust execution of high-throughput workflows.

Boston, Massachusetts, United States

Boston, Massachusetts, United States

South San Francisco, California, United States

San Francisco Bay Area

Project: Plant Media DevelopmentScaled-down cell culture platform to enable screening for both process engineeringoptimizations and media development at micro-titer and micro-bioreactor scale.Developed high-throughput media component screening to optimize cell growth media.Collaborated with Bioprocess Team to ensure successful transfer of optimized conditions.Programmed, operated and troubleshot Hamilton robotic systems.Managed one direct report.

San Francisco Bay Area

Project: Protein Discovery & ScreeningDeveloped and validated a high-throughput screening platform designed to target novel plant-based proteins for use in food products.Developed HT assay that determines protein quality which became a key tool used by Process Team to evaluate isolated protein functionality on a bench/pilot/full scale.Collaborated with Data Scientists to visualize molecular characterization features.Scaled-down assays to 96-well plates to improve throughput and data quality.Automated high-throughput assays that allow the molecular characterization of protein with robust dataset.

Charlottesville, Virginia Area

Project: Protein Purification, Expression, and Crystallization- Expressed, purified, and crystalized target proteins in bacteria, aiming for an in-depthunderstanding of structure-function relationships.- Characterized recombinant proteins with biophysical techniques and functional assays.- Techniques used: X-ray crystallography (27 PDB deposited structures), primary crystalstructure analysis, multiple sequence alignment, Mosquito, RIGAKU, BLAST.

Charlottesville, Virginia Area

Research Project: Expression, purification and crystallization of two proteins in the Aldo-Keto Reductase (AKR) superfamily.Techniques used: bacterial cell culture, immobilized metal affinity chromatography (IMAC), fastprotein liquid chromatography (FPLC), native gel electrophoresis, substrate screening, enzymekinetics, dynamic light scattering (DLS), isothermal titration calorimetry (ITC).

Amsterdam Area, Netherlands

Research Project: Increasing proteasome activity using small molecule compounds.Techniques used: mammalian cell culture, protein gel electrophoresis (SDS-PAGE), western blot, enzyme-linked immunosorbent assay (ELISA), DNA isolation, polymerase chain reaction (PCR),flow cytometry, immunofluorescence, digital microscopy.

Amsterdam Area, Netherlands

Project: Pharmaceutical development- Isolated and purified cannabinoid acids (THCa, CBDa, CBGa) from plant material usingflash chromatography.- Optimized 10 cannabinoids separation using reversed-phase chromatography (RP-HPLC).- Determined the stability of the cannabinoid reference standards for commercial use.

Lodz, Lodz District, Poland

P&G and Global Business Services (GBS) promotion among students and organization of P&G stands, adverts, on-campus presentations.

Master'S Degree, Biomolecular Sciences

Second research project: Expression, purification and crystallization of two members of Aldo-Keto Reductase (AKR) superfamily. First.

Bachelor Of Science, Biotechnology

Activities and Societies: Member of ESN EYE Lodz and Academic Sport AssociationThesis: Ecotoxicity monitoring of hydrocarbon contaminated.

Erasmus, Bioengineering