Omar Alani Email and Phone Number

• Designed and conducted experimental protocols to induce epileptogenesis in mouse models via pilocarpine injectionfollowed by characterization of effects• Manipulated gene expression in epileptic mice and characterized effects to elucidate potential therapeutics for epilepsy• Performed PCR analysis to genotype mouse models and Western Blot analysis to quantify protein levels in mouse models• Detected epileptic seizures in mouse models through analysis of video EEG data• Spearheaded a computer vision project to analyze epileptic mouse behavior, utilizing Deep Lab Cut to train mouse models

•Designed and conducted experiments in human iPSC-derived microglia exploring small molecule modulators of neuroinflammation, relevant to many neurodegenerative diseases• Constructed complex co-culture systems containing human iPSC-derived neural cell types (neurons, astrocytes, microglia) for use in in vitro assays exploring optimization of inflammatory culture conditions• Analyzed neuroinflammatory and target engagement readouts via qPCR and immunocytochemistry, leveraging understanding of Applied Biosystems and Columbus software for quantification of these readouts• Presented and communicated findings to the entire biology division of the company