Pablo Arroyo Email & Phone Number

San Diego, California, Us

Poway, Ca, Us

● Responsible for testing antibody products thru western blotting● Preparing materials for western blots - not limited to cell lysates, SDS-PAGE, and nitrocellulose membranes transfers● Frequently assisting the research team with generating of llama nanobodies, including processing PBMC cells for RNA and cDNA, phage display, and library screening with E. Coli● Running ELISAs (serum titer, inhibition, etc.) for identifying relevant single domain antibodies (sdABs)● Amplification of sdABs thru large scale culturing and induction with IPTG● His-tagged protein purification and concentration of sdABs● Electronically updating antibody product information (including immunocyto/histochemistry and western blot data)

La Jolla, Ca, Us

● Expanded repertoire of culturing techniques to include growing micro aerobes and anaerobes● Routinely maintained and utilized crucial lab equipment including anaerobic and aerobic chambers, lyophilizers, and water baths● Purified bacterial mRNA reads from metatranscriptomic data derived from CF patient data using the bioinformatic program SortMeRNA● Optimized growth of an unidentified siderophore from Rothia mucilaginosa through colorimetric siderophore assays, carbon source growth curves, and increasing surface area for bacterial growth● Isolated siderophores from Rothia mucilaginosa and dentocariosa through techniques including HPLC, AKTA, GC-MS/MS● Adjusted an existing in vitro biofilm model for specifically growing Streptococcus mutans B04Sm5 growth in microbe communities derived from saliva

San Diego, Ca, Us

● Led two different undergraduate courses: Lower division "Introductory Microbiology Lab" and Upper division "Biochemistry, Cell and Molecular Biology Laboratory I".● Presented beginning class lectures ranging from 10-30 minutes on material relating to lab exercises to a maximum audience of 32 students.● Created my own quizzes that utilized the material taught from both lectures, reading materials, and lab exercises.● Taught include bacterial identification and isolation, DNA cloning, and bacteriophage characterization.

San Diego, Ca, Us

Title of my thesis: "Determining the organizational and activational effects of hyperandrogenism in a letrozole-induced mouse model of polycystic ovary syndrome"● Practiced rodent handling skills including scruffing, genotyping, and breeding mice● Mastered invasive in vivo techniques including subcutaneous drug administration, anesthesia, fecal collections, tissue and blood collection, glucose tolerance and insulin tolerance tests, minor animal surgery, estrous cycling● Worked with mice fecal samples to explore the gut microbiome that included extracting bacterial DNA from feces, PCR amplifying the 16s rRNA gene from resulting microbial DNA● Experienced in NGS library preparation and sequencing using an Illumina MiSeq System.● Analyzed 16s rRNA gene from 224 fecal samples thru bioinformatic programs such as QIIME1/2 and Phyloseq● Developed bioinformatics skills such as coding thru R and python, and navigating linux terminals● Learned statistical methods such as repeated measures ANOVA for analyzing differences for multivariable microbiome data● Verified bioinformatic data and physiological data using statistical programs JMP, R, and excel. ● Obtained public presentation skills thru lab meetings, CSUPERB, and thesis defense.

San Diego, Ca, Us

Supported multiple courses for Introductory Microbiology Lab by making all bacterial and yeast cultures that were used. Mastered basic microbiology skills including inoculation and isolating microbes in aseptic conditions, sterilizing and preparing media.

● Overarching focus was on a letrozole-induced mouse model of polycystic ovary syndrome.● Developed skills in animal husbandry with mice (scruffing, cage changes, genotyping, euthanasia).● Exposed to more invasive mice handling, including subcutaneous injections with diluted insulin or glucose, collecting blood thru tail snips, and surgical implantations of letrozole.● Performed bacterial DNA extractions of mice fecal samples● Assisted graduate students with post-sac mouse blood and tissue collections

La Jolla, Ca, Us

● Part of the UCSD Summer Academy for Transfer Students (currently PATH).● This was my first exposure to working with incoming undergraduate students from a teaching point of view.● Worked with a class of 17 students in their laboratory class: Biochemical Techniques● Provided mini-lectures for daily experiments● Supervised students with common laboratory techniques (SDS-PAGE, Protein purification columns, )● Proctored exams and quizzes● Tutored students twice a week with lab material including dilutions problems, lab scenarios, and concentration equations.● Provided mentoring to students by taking them out for lunch weekly, mainly focusing on topics relating to undergraduate life at UC San Diego.

Master'S Degree, Microbiology, General

Bachelor’S Degree, Physiology And Neuroscience