Patrick De Jonge Email & Phone Number

Amsterdam, North Holland, Netherlands

Delft, Netherlands

From September 2016 up to November 2019, I was a guest researcher at the Department of Bionanoscience at TU Delft. Here I performed the wetlab experiments incorporated in my PhD research. During this time I supervised two students during their internships, and was a teacher's assistant for the 2017 TU Delft iGEM team.

Utrecht, Netherlands

My PhD research focused on studying the interactions between phages and their bacterial hosts, in particular in the human gastro-intestinal tract. We studied the evolution and biodiveristy of probable phage host-binding mechanisms in silico. We further worked to develop a methodology for high-throughput analysis of phage-host interactions. This project gave me working experience with metagenomic sequencing, electron microscopy, and chemical polymer synthesis, among others. I performed my PhD at the Theoretical Biology and Bioinformatics department of Utrecht University. Meanwhile, I was a guest researcher at the Laboratory for Microbiology at Wageningen University up to September 2016, and at the Department of Bionanoscience at TU Delft between September 2016 and November 2019.

Wageningen, Netherlands

For the first year of my PhD, I performed wetlab experiments as a guest researcher at the Laboratory for Microbiology at Wageningen UR. During this time I was also a teachers' assistant for the Bachelor introductory microbiology course.

Rotterdam, Netherlands

The ResearchAnt Foundation is a non-profit organisation which aims to facilitate drug development, especially antibiotics, through a compound exchanging system. Within this organisation I am responsible for all scientific aspects of an antibiotics database:• Conducted manual validation of a database containing 5,000 antimicrobial compounds and over 8,000 unique microbial strains.• Overhauled a database of over 650 plasmids and related growth conditions.• Designed protocols for the identification of approximately 750 new data sources from a large body of scientific literature.• Tutored students in data-mining efforts

Ede, Netherlands

- Executed microbiological tests in a production environment.- Responsible for all activities in a laboratory department.- Independently assessed and verified data from microbial food-safety tests

Nijmegen, Netherlands

- Title: Heterologous expression of putative nitrite reductases in Methanosarcina acetivorans.- Executed gene transfer from uncultured Archaea to a methanogen, creating several mutant strains.- Independently coordinated research between two academic departments.- Established the infrastructure to culture methanogens for gene expression studies.- Key techniques: anaerobic culturing, GC, in silico gene expression, molecular biology.

Dresden, Germany

Wageningen, Netherlands

- Title: Enrichment and isolation of butyrate degrading microbes from marine methanogenic sediments.- Established enrichment cultures for three players in a marine food web.- Analysed microbial populations with biochemical, molecular and microscopic tools.- Key techniques: GC, HPLC, DGGE, microscopy, anaerobic culturing.

Zürich, Switzerland

- Title: Physiology of Acetyl-CoA assimilation in Paracoccus denitrificans.- Improved culturing conditions to study microbial activity under aerobic vs. anaerobic conditions.- Established two new enzyme assays to examine activity for several key enzymes.- Key techniques: 13C labelling experiments, enzyme assays, culture optimisation.

Nijmegen, Netherlands

- Title: Heterologous expression of the large subunit of RuBisCO from ‘Ca. Methylomirabilis oxyfera’.- Key techniques: Heterologous expression, MALDI-TOF.

Bangor, Uk

Wageningen, Netherlands

Doctor Of Philosophy - Phd, Microbiology, Metagenomics, And Phage Biology

Master’S Degree, Microbiology, Judicium Bene Meritum

Bachelor Of Applied Science (B.A.Sc.), Life Sciences

Laboratory Analist, Molecular Biology