Pamy Noldner Email and Phone Number

Technical lead for the development of procedures and protocols for the manufacture of various cell products starting with cell isolation from source material to large scale expansion, cryopreservation and final formulation. Writing SOPs and manufacturing forms and train GMP staff on new procedures.Evaluate bioreactors and cell processing devices in order to streamline manufacturing of cell therapy products as well as improve process capabilities and closely collaborated with the vendors to tailor their product to our requirements.Publications:Sotland P, Buntz S, Noeldner P, Saha A, Gentry T, Kurtzberg J, Balber A (2017)Gene products promoting remyelination are up-regulated in a cell therapy product manufactured from banked human cord blood.CytotherapySaha A, Buntz S, Scotland P, Xu L, Noeldner P, Patel S, Wollish A, Gunaratne A, Troy J, Matsushima GK, Kurtzberg J, Balber A (2016)A cord blood monocyte-derived cell therapy product accelerates brain remyelinationThe Journal of Clinical Investigation, 1(13):e86667Shoulars K, Noldner P, Troy JD, Cheatham L, Parrish A, Page K, Gentry T, Balber A, Kurtzberg J (2016)Development and validation of a rapid, aldehyde dehydrogenase bright-based cord blood potency assayBlood, 127:2346-2354Kurtzberg J, Buntz S, Gentry T, Noeldner P, Ozamiz A, Rusche B, Storms RW, Wollish A, Wenger DA, Balber AE (2015)Preclinical characterization of DUOC-01, a cell therapy product derived from banked umbilical cord blood for use as an adjuvant to umbilical cord blood transplantation for treatment of inherited metabolic diseases.Cytotherapy, 17(6): 803-15

• Design and cloning of a DNA targeting construct to create a gene-deleted mouse. Characterization of the mouse phenotype (gross anatomy, tissues, cell types)• Described and studied the effect of genetic ablation of CaMKK1 in T-cell development in thymus. Immunophenotype analysis: apoptosis, proliferation, differentiation.• Developed an in-vitro peptide-kinase assay for screening a compound library for specific inhibitory activity towards Ca/Calmodulin-dependent kinases. (EC50s, Graphpad Prism software)• Studied protein-protein interaction: designed and cloned a variety of protein mutants and mapped interacting domains by ectopically expressing mutants in cells followed by co-immunoprecipitation.• Studied the role of CaMKIV in the regulation of CREB-mediated transcriptionPUBLICATIONS:Sankar U, Noeldner P, Camp J, Wilkerson D, Jonsson C, Anderson K, Means A, Racioppi L (2017)CaMKK1 regulates thymus development and immunosenescenceScientific Reports (submitted)Racioppi, L, Noeldner PK, Lin F, Arvai S, Means AR (2012) Calcium/Calmodulin-Dependent Protein Kinase Kinase 2 Regulates Macrophage- Mediated Inflammatory Responses. JBC 287:11579-11591Anderson KA, Ribar TJ, Lin F, Noeldner PK, Green MF, Muehlbauer MJ, Witters LA, Kemp BE, Means AR (2008)Hypothalamic CaMKK2 contributes to the regulation of energy balance. Cell Metab. 7 (5): 377–88Chow FA, Anderson KA, Noeldner PK, and Means AR (2005)The autonomous activity of calcium/calmodulin-dependent protein kinase IV is required for its role in transcription. JBC 280(21):20530-8Anderson KA, Noeldner PK, Reece K, Wadzinski BE, and Means AR (2004)Regulation and function of the calcium/calmodulin-dependent protein kinase IV/protein serine/threonine phosphatase 2A signaling complex. JBC 279(30):31708-16Lemrow, SM, Anderson, KA, Joseph, JD, Ribar, TJ, Noeldner, PK, Means, AR (2004)Catalytic activity is required for CaMKIV to enter the nucleus. JBC 279:11664-11671