Ashutosh K. Pandey, Ph.D. Email and Phone Number
Ashutosh K. Pandey, Ph.D. work email
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Ashutosh K. Pandey, Ph.D. personal email
9+ years of research experience in the field of chemistry, biochemistry, and molecular biology methods and techniques, including bioassay development, pharmaceutical drug testing, peptides or small molecule discoveries, genetics, recombinant protein purification and characterization, biomarker discovery, Mitochondrial/cytoplasmic iron-sulfur cluster biogenesis, protein import, tRNA thiolation and metal-ion homeostasis in human, yeast, bacteria, and mammalian cells for cancer, metabolic and neurodegenerative diseases. 2+ years of practical experience in viral DNA cloning and transformation, as well as protein isolation. During this time, I have focused on identifying mechanisms associated with the Hepatitis C virus and Human immunodeficiency virus type 1 (HIV-1), contributing to advancements in understanding these viral pathogens and their impact on human health.2+ years of research experience specializing in multistep drug molecule synthesis and subsequent characterization. This involves a comprehensive understanding of synthesizing complex molecules through multiple sequential reactions and thoroughly characterizing them to assess their chemical properties and potential applications in drug development.Technical Expertise • Size exclusion chromatography (SEC) • Thin-layer chromatography (TLC) • Gas chromatography (GC) • Fast-protein liquid chromatography (FPLC) • High-performance liquid chromatography (HPLC) • UV-visible spectrophotometry • Seahorse XF analyzer • Plate reader • Microscopy • Gel electrophoresis • Western blot • Cell culture • PCR • PyMOL • Mitochondria & cytoplasm isolations • E. coli • Immunoprecipitations (IP) • Enzyme kinetics • Radiolabeling of proteins & nucleotides • Fluorescence and luminescence assays • In-vitro protein-protein & protein-DNA interactions • Graphpad prism • Adobe photoshop & Illustrator • Statistical analysis & data processing • Microsoft office Skills • Demonstrated ability to multitask • Excellent written and communication skills • Good teamwork skills • Excellent problem-solving skills • Strong leadership abilities • Experimental design and data analysis • Scientific communications • Ability to handle multiple projects • Technical troubleshooting
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Staff Scientist IAtek Inc-Nj Public Health & Environmental LaboratoriesNewark, Nj, Us
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Senior Research Associate ScientistRutgers New Jersey Medical School Nov 2017 - Present185 South Orange Ave, Newark, New Jersey, Usa• Innovated novel chemical and biochemical assays to identify specific drug molecules, peptides, or proteins in yeast (saccharomyces cerevisiae), Bacteria (E. coli) Human (fibroblast) and Mammalian cells (HeLa, HEK293 & CAD) are critical for addressing cancer, metabolic disorders, and neurodegenerative diseases.• Utilized advanced techniques such as FPLC, HPLC, UV-visible spectrophotometry, LC-MS, and microscopy to ensure precise results in developing biochemical assays spanning bacteria, yeast, and mammalian cells.• Designed and purified several bacterially expressed proteins (soluble/inclusion body/radiolabeled) using affinity, ion exchange, and size-exclusion chromatography systems.• Examined the function of proteins in binding, proteolysis, folding, import processes, enzyme kinetics, activity assay, and iron-sulfur protein cluster loading as well as their involvement in interaction and regulation.• Developed and evaluated bioassays using Native/SDS-PAGE electrophoresis, western blotting, immunoprecipitation, immunoblotting, and UV-visible spectrophotometry techniques, ensuring accurate results across cellular contexts.• Presented the progress and findings of our team and disseminated our research results through publication in peer-reviewed international journals.• Trained 5-6 junior scientists or volunteers, ensuring unity and coordination in laboratory projects. -
Postdoctoral Research ScientistRutgers New Jersey Medical School Nov 2012 - Oct 2017Newark, New Jersey, United States• Employed sophisticated methodologies including FPLC, Seahorse XF analysis, and UV-visible spectrophotometry to guarantee accurate outcomes during the creation of biochemical assays.• Participated in isolating mitochondrial proteins from bacteria (E. coli), purified by Ni-NTA agarose followed by FPLC, and checked its monomeric/dimeric properties by HPLC.• Engaged in elucidating the drug resistance mechanism of mitochondrial proteins using various triterpenoid drug compounds for the development of therapeutic bioassays.• Investigated the roles of proteins (endogenous/recombinant) in binding, proteolysis, folding, import processes, enzyme kinetics, inhibition, and their involvement in interaction and regulation.• Involved in the investigation of the impact of acetylation and phosphorylation on a human protein-DNA interaction, employing contrasting mechanisms to identify potential biomarkers.• Created and executed scenarios for analysis projects and interpreted results to address relevant questions.• Guided 6-7 junior members, ensuring cohesion and coordination in laboratory projects.
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Research ScientistCheminova India Limited Aug 2010 - Sep 2012Ankaleshwar, Gujarat, IndiaThrough my dedication to research and commitment to excellence, I played a crucial role in achieving scientific objectives and organizational milestones.• Led a committed team of 5-6 individuals, fostering cohesion and alignment in laboratory projects.• Took part in a team focused on conducting R&D experiments, qualitative and quantitative analyses, daily experimentation, and documenting results by cGMP standards.• Conducted laboratory experiments utilizing GC and HPLC instrumentation along with a range of chemistry techniques such as organic synthesis, titration, crystallization, purification, distillation, separation, column chromatography, thin layer chromatography, extraction, and dilutions.• Adjusted and customized standard methods and procedures to address analytical challenges effectively.
Ashutosh K. Pandey, Ph.D. Skills
Ashutosh K. Pandey, Ph.D. Education Details
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V.B.S Purvanchal University/Banaras Hindu University, IndiaInorganic Chemistry
Frequently Asked Questions about Ashutosh K. Pandey, Ph.D.
What company does Ashutosh K. Pandey, Ph.D. work for?
Ashutosh K. Pandey, Ph.D. works for Atek Inc-Nj Public Health & Environmental Laboratories
What is Ashutosh K. Pandey, Ph.D.'s role at the current company?
Ashutosh K. Pandey, Ph.D.'s current role is Staff Scientist I.
What is Ashutosh K. Pandey, Ph.D.'s email address?
Ashutosh K. Pandey, Ph.D.'s email address is as****@****ers.edu
What schools did Ashutosh K. Pandey, Ph.D. attend?
Ashutosh K. Pandey, Ph.D. attended V.b.s Purvanchal University/banaras Hindu University, India, V.b.s Purvanchal University, India, V.b.s Purvanchal University, India.
What are some of Ashutosh K. Pandey, Ph.D.'s interests?
Ashutosh K. Pandey, Ph.D. has interest in Social Services, Children, Education, Environment, Science And Technology, Human Rights, Arts And Culture, Health.
What skills is Ashutosh K. Pandey, Ph.D. known for?
Ashutosh K. Pandey, Ph.D. has skills like R, Hplc, Chemistry, Nmr, Uv/vis, Organic Chemistry, Organic Synthesis, Analytical Chemistry, Chromatography, Mass Spectrometry, Biochemistry, Spectroscopy.
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