Ashutosh K. Pandey, Ph.D. Email & Phone Number

Newark, Nj, Us

Newark, Nj, Us

185 South Orange Ave, Newark, New Jersey, Usa

• Innovated novel chemical and biochemical assays to identify specific drug molecules, peptides, or proteins in yeast (saccharomyces cerevisiae), Bacteria (E. coli) Human (fibroblast) and Mammalian cells (HeLa, HEK293 & CAD) are critical for addressing cancer, metabolic disorders, and neurodegenerative diseases.• Utilized advanced techniques such as FPLC, HPLC, UV-visible spectrophotometry, LC-MS, and microscopy to ensure precise results in developing biochemical assays spanning bacteria, yeast, and mammalian cells.• Designed and purified several bacterially expressed proteins (soluble/inclusion body/radiolabeled) using affinity, ion exchange, and size-exclusion chromatography systems.• Examined the function of proteins in binding, proteolysis, folding, import processes, enzyme kinetics, activity assay, and iron-sulfur protein cluster loading as well as their involvement in interaction and regulation.• Developed and evaluated bioassays using Native/SDS-PAGE electrophoresis, western blotting, immunoprecipitation, immunoblotting, and UV-visible spectrophotometry techniques, ensuring accurate results across cellular contexts.• Presented the progress and findings of our team and disseminated our research results through publication in peer-reviewed international journals.• Trained 5-6 junior scientists or volunteers, ensuring unity and coordination in laboratory projects.

Newark, New Jersey, United States

• Employed sophisticated methodologies including FPLC, Seahorse XF analysis, and UV-visible spectrophotometry to guarantee accurate outcomes during the creation of biochemical assays.• Participated in isolating mitochondrial proteins from bacteria (E. coli), purified by Ni-NTA agarose followed by FPLC, and checked its monomeric/dimeric properties by HPLC.• Engaged in elucidating the drug resistance mechanism of mitochondrial proteins using various triterpenoid drug compounds for the development of therapeutic bioassays.• Investigated the roles of proteins (endogenous/recombinant) in binding, proteolysis, folding, import processes, enzyme kinetics, inhibition, and their involvement in interaction and regulation.• Involved in the investigation of the impact of acetylation and phosphorylation on a human protein-DNA interaction, employing contrasting mechanisms to identify potential biomarkers.• Created and executed scenarios for analysis projects and interpreted results to address relevant questions.• Guided 6-7 junior members, ensuring cohesion and coordination in laboratory projects.

Ankaleshwar, Gujarat, India

Through my dedication to research and commitment to excellence, I played a crucial role in achieving scientific objectives and organizational milestones.• Led a committed team of 5-6 individuals, fostering cohesion and alignment in laboratory projects.• Took part in a team focused on conducting R&D experiments, qualitative and quantitative analyses, daily experimentation, and documenting results by cGMP standards.• Conducted laboratory experiments utilizing GC and HPLC instrumentation along with a range of chemistry techniques such as organic synthesis, titration, crystallization, purification, distillation, separation, column chromatography, thin layer chromatography, extraction, and dilutions.• Adjusted and customized standard methods and procedures to address analytical challenges effectively.

Ph.D., Inorganic Chemistry

Research area: “Synthesis and characterization of organometallic compounds and polymerization.”

Master'S Degree, Chemistry, First

Bachelor'S Degree, Chemistry And Biology, First