Ashutosh K. Pandey, Ph.D. Email & Phone Number

Newark, NJ, US

185 South Orange Ave, Newark, New Jersey, USA

• Innovated novel chemical and biochemical assays to identify specific drug molecules, peptides, or proteins in yeast (saccharomyces cerevisiae), Bacteria (E. coli) Human (fibroblast) and Mammalian cells (HeLa, HEK293 &.
• Utilized advanced techniques such as FPLC, HPLC, UV-visible spectrophotometry, LC-MS, and microscopy to ensure precise results in developing biochemical assays spanning bacteria, yeast, and mammalian cells.
• Designed and purified several bacterially expressed proteins (soluble/inclusion body/radiolabeled) using affinity, ion exchange, and size-exclusion chromatography systems.
• Examined the function of proteins in binding, proteolysis, folding, import processes, enzyme kinetics, activity assay, and iron-sulfur protein cluster loading as well as their involvement in interaction and regulation.
• Developed and evaluated bioassays using Native/SDS-PAGE electrophoresis, western blotting, immunoprecipitation, immunoblotting, and UV-visible spectrophotometry techniques, ensuring accurate results across cellular.
• Presented the progress and findings of our team and disseminated our research results through publication in peer-reviewed international journals.

Newark, New Jersey, United States

• Employed sophisticated methodologies including FPLC, Seahorse XF analysis, and UV-visible spectrophotometry to guarantee accurate outcomes during the creation of biochemical assays.
• Participated in isolating mitochondrial proteins from bacteria (E. coli), purified by Ni-NTA agarose followed by FPLC, and checked its monomeric/dimeric properties by HPLC.
• Engaged in elucidating the drug resistance mechanism of mitochondrial proteins using various triterpenoid drug compounds for the development of therapeutic bioassays.
• Investigated the roles of proteins (endogenous/recombinant) in binding, proteolysis, folding, import processes, enzyme kinetics, inhibition, and their involvement in interaction and regulation.
• Involved in the investigation of the impact of acetylation and phosphorylation on a human protein-DNA interaction, employing contrasting mechanisms to identify potential biomarkers.
• Created and executed scenarios for analysis projects and interpreted results to address relevant questions.

Ankaleshwar, Gujarat, India

• Through my dedication to research and commitment to excellence, I played a crucial role in achieving scientific objectives and organizational milestones.
• Led a committed team of 5-6 individuals, fostering cohesion and alignment in laboratory projects.
• Took part in a team focused on conducting R&D experiments, qualitative and quantitative analyses, daily experimentation, and documenting results by cGMP standards.
• Conducted laboratory experiments utilizing GC and HPLC instrumentation along with a range of chemistry techniques such as organic synthesis, titration, crystallization, purification, distillation, separation, column.
• Adjusted and customized standard methods and procedures to address analytical challenges effectively.

Ph.D., Inorganic Chemistry

Research area: “Synthesis and characterization of organometallic compounds and polymerization.”

Master'S Degree, Chemistry, First

Bachelor'S Degree, Chemistry And Biology, First