Partha Sampathkumar Email & Phone Number

South San Francisco, California, United States

California, United States

• Produced several “difficult to express and purify” proteins (at 1- to 10- liter scale) with low endo-toxin for in vivo immunization campaigns and structural studies.
• Proficiently applied AKTA systems for protein purification process development using affinity (ProA, His-tag, and Strep-tag), ion-exchange, size-exclusion, ceramic hydroxyapatite, and hydrophobic chromatography methods.
• Received “Upside-Down” and “Figure 8” awards at Surrozen.
• Lead author in a manuscript (soon to be submitted) highlighting the structure and function of Surrozen’s SWEETS molecules as antibody-based PROTACs that function as a Targeted Protein Degradation system via AbTAC and.
• Led generation of Developability Data Package that were critical to selection of Surrozen’s clinical candidates SZN-413, SZN-043, and SZN-1326 for Retinal, Liver, and Intestinal diseases.
• Led and performed Developability assays for Biologics including thermal stability, HIC, computational sequence- and structure-based Manufacturability assessments, Forced-degradation, AAPH-oxidation stress, low pH-hold.

San Francisco Bay Area

• Determined ~20 crystal structures of antigen-Fab complexes for epitope mapping, structure activity relationships (SAR) and analyzed structures for protein engineering and development of biologics.
• Patent: WO2019126399 - ANTI-FRIZZLED ANTIBODIES AND METHODS OF USE
• Patent: WO2019126401 - ANTI-LRP5/6 ANTIBODIES AND METHODS OF USE
• Patent: WO2020132356 - ANTIGEN BINDING FORMATS FOR RECEPTOR COMPLEXES
• Patent: WO2021003416 - MONOSPECIFIC ANTI-FRIZZLED ANTIBODIES AND METHODS OF USE
• Patent: WO2022192445 - MODULATION OF WNT SIGNALING IN GASTROINTESTINAL DISORDERS

San Francisco Bay Area

New York NY

• Purification, crystallization, and structure determination of human Jak2 pseudokinase domain with small-molecule ligands, and expression of full-length and 3-doamin human Jak2 in Baculovirus-insect cell system..

New York, NY

• Extensively applied baculovirus / insect-cell system for expression of a human kinase towards purification, characterization, and crystallization trails.
• Transiently expressed kinase mutants in HEK293 cells in 96-well format and characterized their expression and activation using western blot analyses.
• Performed gene cloning, small-scale expression testing, large-scale protein production, crystallization screens, and crystal-growth optimization using high-throughput techniques.
• Guided associate scientists in day-to-day laboratory research and involved in project management.

Greater New York City Area

• The Nuclear Pore Complex, composed of proteins called Nucleoporins (Nups), mediates the nucleo-cytoplasmic transport of macromolecules in eukaryotes. Determined the structure of a 110 kDa N-terminal domain S..
• Determined the high-resolution structure of mouse CD27, a Tumor Necrosis Factor (TNF) receptor, by Sulfur-phasing, and currently working towards structure determination of CD27 with its TNF-ligand CD70.
• Successfully refolded mouse and human Leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1; a collagen receptor), and determined their high-resolution crystal structures by SeMET-SAD phasing.Publications:1..

San Diego CA

• PHR proteins play a critical role in synaptogenesis and axon guidance. PHR domains are essential for proper localization of PHR proteins. Determined first high-resolution crystal structures PHR domains of Mouse Phr-1.
• Determined and analyzed the crystal structure of an integral membrane protein BenF-like porin from Pseudomonas fluorescens.
• Obtained hands-on experience in purification, characterization, and setting up lipidic cubic phase (LCP) crystallization of membrane proteins.
• Performed Construct Design using a variety of bioinformatics tools, and high-throughput molecular cloning, expression, and purification of several proteins.
• Contributed towards structure determination of several Protein Structure Initiative project targets, and played a leadership role in writing and communicating research papers to scientific journals as corresponding.

• PHR proteins play a critical role in synaptogenesis and axon guidance. PHR domains are essential for proper localization of PHR proteins. Determined first high-resolution crystal structures PHR domains of Mouse Phr-1.
• Determined and analyzed the crystal structure of an integral membrane protein BenF-like porin from Pseudomonas fluorescens.
• Hands-on experience in purification and lipidic cubic phase (LCP) crystallization of membrane proteins.
• Performed Construct Design using a variety of bioinformatics tools, and high-throughput molecular cloning, expression, and purification of several proteins.
• Contributed towards structure determination of several Protein Structure Initiative project targets and played a leadership role in writing and communicating research papers to scientific journals as corresponding.

• Peroxins (PEX) from Trypanosoma brucei:
• Successfully crystallized the Peroxisomal Targeting Signal 1 (PTS1) binding domain of Trypanosoma brucei PEX5, a potential drug target, by extensively application of Surface Entropy Reduction mutagenesis.
• Determined crystal structures of T. brucei PEX5 in complex with five different PTS1 peptides, thereby identifying a unique hydrophobic pocket in the subdomain interface that represents an attractive inhibitor binding.
• Determined the crystal structure of Mycobacterium tuberculosis ThyX (~2000 residues in the asymmetric unit), a potential drug target responsible for synthesis of thymidine, in the presence of the co-factor FAD and a.
• Determined structure of MtbThyX-NADP+ complex and obtained insights to design bi-functional inhibitors.Publications:1. Sampathkumar P, Roach C, Michels PAM and Hol WGJ (2008). Structural insights into recognition of.

Ph.D., Biophysics, Structural Biology

Activities and Societies: • Received all India Rank 3 (score of 99.81 percentile) in the Graduate Aptitude Test in Engineering.

Master Of Science (M.Sc.), Chemistry, First Class With Distinction (Gold Medal)

Bachelor Of Science (B.Sc.), Chemistry, Physics, And Mathematics, First Class