• Develop an ELISA platform for High Throughput screening of target proteins.• Develop an In-vitro system to study protein-protein interactions.• Screen Small molecule PROTACS and generate Dose Response Curves in Mammalian cells.• Isolate PBMCs and monocytes for Cytokine analysis using reporter assays.• Transient and Stable transfection to generate disease relevant Cell lines.• Perform qPCR and FACs to validate cell lines. • Perform Mini preps for isolation and purification of Plasmids.• Perform Immunoprecipitations using polyclonal antibodies and Small molecule conjugated beads.• Dose, Lyse, Desalt, TMT label and fractionate samples for fingerprinting using LC/MS.

• Treat and screen Protacs for Efficacy using High throughput cell based degradation Assay’s.• Develop an ELISA platform for High Throughput Screening of target proteins.• Transfect Mammalian Cells using plasmid DNA.• Perform Immunoprecipitations using polyclonal antibodies to Isolate target proteins.• Dose, Lyse, Desalt, TMT label and fractionate samples for fingerprinting using LC/MS.• Assay optimization to increase throughput and accuracy of In-house Cellular Assays.

Research Co-op, Momenta Pharmaceuticals, Cambridge, MA January 2017- June2017 • DOE to Scout HIC and SCX resins using AKTA Pure 25.• Write and execute various methods for HIC Purification. Which resulted in a single component product. • Analyze purified probes using SEC and SDS-PAGE.• Assist in Protein A, SCX, and Multicolumn purifications.• Endotoxins testing of probes and systems using LAL test.• Collaborate with the team to pack purification columns.• Desalt and concentrate Protein solutions.• Study Effect of Depth filtration on highly concentrated IVIG solutions.• Evaluate various colorimetric protein kits from G-Biosciences for protein quantification.• Perform Western Blots to detect presence of ubiquitinated proteins.• Helped in tech transfer to CMO.

• Purification of His tagged proteins.• Protein Expression in PANC-1 cells and sf9 cells.• SDS-PAGE and Western Blot analysis.• Perform Silver Staining, Coomaisse stain, and Ponceau dye stain.• Isolate, Purify and quantify RNA. Execute RT-PCR to prepare cDNA. And Perform qPCR• Perform ELISA to detect cytokines.• Prepare and Run Agarose Gel to Genotype mice.• Handle and collect serum samples from mice

Helped maintain inventory control records and export records in warehouse and B.S.R. Assisted Manufacture Department for Tablet, Capsule, Injectables. Learned and helped in the manufacturing of the formulations. Learned about Maintaining B.M.R records for manufactured batches. Worked on Quality Control, Conducted Quality Control tests and tabulated results. Trained in Quality Assurance Department and helped make S.O.P for cleaning manufacturing area.