Patrick Aghajanian, Phd Email & Phone Number

Project: Genetic Curation and Gene Copy Number Analysis• Became integral for the initial determination of likely copy number variations using internal NGS analysis tools.• Provided quality assurance testing and refinement of multiple different testing platforms at Fulgent.• Curated and wrote reports for hundreds of panels including comprehensive epilepsy, intellectual disability, parental carrier, known mutation and whole exome panels.• Wrote standard operating protocols for different testing platforms and copy number analysis.• Analyzed genes to further streamline future copy number analysis via automation.• Regularly worked with other departments to verify accurate results and test specifications for client reports.• Trained multiple individuals in genetic curation and copy number analysis.• Aided in the testing of COVID-19 patients in the laboratory and establishment of testing sites in Los Angeles county.

Loma Linda, Ca

Project: Epiphyseal Transdifferentiation of Chondrocytes to Osteoblasts• Examined the effect of ascorbic acid and thyroid hormone on bone and cartilage cell lines and primary cells via qPCR analysis and immunocytochemistry.• Analyzed expression of various bone and cartilage markers via fluorescent microscopy.• Performed immunohistochemical assays on a variety of transgenic mouse bones to determine lineage specificity and hormonal effects.• Analyzed protein-promoter binding via gel-shift assays.• Spoke at two conferences to relay scientific research findings to colleagues through poster presentations.

Westwood, Ca

Project: Development of Intestinal Stem Cells and Visceral Muscle during Drosophila Metamorphosis• Designed and used genetic cross schemes for targeted muscle ablation and expression of endogenous fluorescent proteins in transgenic flies.• Dissected animals at varying stages of development and removed whole organs for analysis.• Used immunohistochemical staining against various developmental markers to determine the existence of niches, stem cells and terminally differentiated cells.• Performed 3D image analysis of whole tissues via confocal microscopy Z-stacking.

Westwood, Ca

Project: Antibody engineering, expression and therapeutics for Prostate Cancer• Assayed antibody fragments for therapeutic potential on prostate cancer cell lines and analyzed their effects via MTT, Annexin V and trypan blue assays.• Performed a large number of molecular cloning experiments, analysis and protein expression/purification.• Worked with senior scientists and graduate students to develop antibody fragments to be used in PET scans.

Doctor Of Philosophy (Ph.D.), Molecular, Cell, And Developmental Biology

Bs, Molecular, Cell And Developmental Biology

Biology, General