• Automated processing of images using ImageJ.• Developed custom pipelines to automated data analysis. • Enzyme engineering using structural modeling and computational approaches.

• Analyzed 48 illumina RNA and 24 illumina small RNAs libraries.• Created bioinformatics pipelines. • Built infectious clones using Gibson cloning and added a multicloning site.• Demonstrated infectivity and expression of virus infectious clone in Fragaria plants.• Generated clean Rubus plant stock using tissue culture.• Treated Rubus plants with antivirals and small RNAs. • Presented findings at national meetings.

• Managed six sections of up to 24 students for four ten-week courses. • Made botany, plant pathology, molecular biology, and bioinformatic course content.• Lectured weekly presentations.

• Isolated RNA and double stranded RNA from plants. • Designed primers for RT-PCR and RT-QPCR for virus detection. • Screened multiple cDNA libraries to obtain 10x sequencing coverage. • Screened inserts using PCR, restriction enzymes and Sanger Sequencing.• Analyzed next generation 454-Junior and illumina data of Rosa and Cercis canadensis.• Performed vector transmission using eriophyid mites and aphids.• Screened roses for virus and eriophyid mite resistance.• Monitored lab’s greenhouse for pests.• Developed scientific policy with an international group based on findings.

• Pioneered a project surveying DC street trees for the pathogen Xylella fastidiosa.• Isolated bacterial DNA, detection via ELISA, PCR, and sequence analysis.• Cultured Oomycetes and fungi and identified using PCR and sequence analysis.• Presented findings at scientific meetings and to stakeholders.

• Isolated plant DNA.• Performed and troubleshooted Amplified fragment length polymorphism (ALFP). • Examined ploidy levels with a flow cytometry.• Presented results at a in unit conference.

• Screened the entomopathogenic Metarhizium fungi against Cyclocephala insect larva.• Assisted in wheat and barley breeding program in the field and greenhouse.• Evaluated harvested grain characteristics.

• Assisted with maintenance of greenhouse plants. • Tested Methylobacterium bacterial strains for their potential to stimulate plant growth. • Created mutant strains via tri-parental mating and tested their ability to metabolize substrates.• Patented a method of using Methylobacterium bacteria to stimulate algae growth.