Patrick L. Email and Phone Number
Molecular biologist with over 10 years of research experience working in gene therapy and diagnostic companies, specialized in Next Generation Sequencing (NGS), genomics, bioinformatics, and genome editing technologies.- Supervised a team of Ph.D. level scientist and research associates- Implemented unbiased genome-wide off-target assays (both in vivo and in vitro) and identified off-targets for internal and external programs- Serve as subject matter expert on gene editing specificity with external partners, FDA, and NIST- Developed various assays for difficult-to-sequence repeat expansion disease genes- Developed assays for somatic NGS panel testing of FFPE tumor tissues- Developed assays for splicing VUS resolution- Drafted SOPs and validation plans- Oversaw the NGS team to provide NGS service and bioinformatic support to research scientists- Established a tissue-specific ribosome-bound mRNA isolation method in Drosophila melanogaster- Identified dietary restriction-induced tissue-specific translation regulation- Uncovered the translation mechanism of mouse L1 retrotransposon- Identified L1 mRNA-binding cellular modulators of retrotransposition- Published over 20 peer-reviewed articles
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Associate Director Of Nucleic Acid TechnologiesJohnson & Johnson Innovative MedicineCalifornia, United States -
Associate Director Of Nucleic Acid TechnologiesJohnson & Johnson Innovative Medicine May 2022 - Present -
Associate Director Of Gene SpecificitySangamo Therapeutics, Inc. Jul 2020 - May 2022Brisbane, California, Us -
Senior ScientistInvitae May 2017 - Jul 2020San Francisco, Ca, Us -
Scientist IiiSangamo Therapeutics, Inc. May 2013 - May 2017Brisbane, California, UsPerformed and supervised NGS run and sequencing data analysis in the context of gene editing.Implemented and executed off-target studies of various gene editing nucleases. -
Postdoctoral Research ScientistBuck Institute Jan 2008 - May 2013Developed technology in Drosophila to isolate tissue-specific ribosome-bound mRNA by immunoprecipitationPerformed and analyzed gene expression studies using RNAseq, microarray and high throughput qRT-PCRGenerated polysome profiles to monitor mRNA translation statusCreated overexpression plasmids using molecular cloning techniquesWrote basic scripts in R to analyze data and generate figures for publication
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Phd StudentUniversity Of Colorado Anschutz Medical Campus Feb 2001 - Dec 2007Us -
Graduate Research ScientistUniversity Of Colorado Anschutz Medical Campus Feb 2001 - Dec 2007UsIdentified and characterized Internal Ribosome Entry Sites (IRES) using luciferase reporter assayPerformed siRNA experiments and verified efficiency by Western blotCultured mammalian cells and performed cell-based assaysCreated reporter constructs using standard molecular cloning techniquesIsolated sequence-specific RNA binding proteinProbed for RNA secondary structureIdentified target sequence of RNA binding proteinMeasured affinity of an RNA binding proteinPurified overexpressed proteins from baculovirus infected cells
Patrick L. Education Details
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University Of Colorado Anschutz Medical CampusHuman Medical Genetics -
Texas A&M UniversityGenetics
Frequently Asked Questions about Patrick L.
What company does Patrick L. work for?
Patrick L. works for Johnson & Johnson Innovative Medicine
What is Patrick L.'s role at the current company?
Patrick L.'s current role is Associate Director of Nucleic Acid Technologies.
What schools did Patrick L. attend?
Patrick L. attended University Of Colorado Anschutz Medical Campus, Texas A&m University.
Who are Patrick L.'s colleagues?
Patrick L.'s colleagues are Pascual Arizmendi, Andy Symes, Arvi D., Jeff Lol, Mina Yanny, Aleksandr Pelts, Lana Kandalaft.
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