Patrick L.

Patrick L. Email and Phone Number

Associate Director of Nucleic Acid Technologies @ Johnson & Johnson Innovative Medicine
California, United States
Patrick L.'s Location
San Francisco Bay Area, United States, United States
About Patrick L.

Molecular biologist with over 10 years of research experience working in gene therapy and diagnostic companies, specialized in Next Generation Sequencing (NGS), genomics, bioinformatics, and genome editing technologies.- Supervised a team of Ph.D. level scientist and research associates- Implemented unbiased genome-wide off-target assays (both in vivo and in vitro) and identified off-targets for internal and external programs- Serve as subject matter expert on gene editing specificity with external partners, FDA, and NIST- Developed various assays for difficult-to-sequence repeat expansion disease genes- Developed assays for somatic NGS panel testing of FFPE tumor tissues- Developed assays for splicing VUS resolution- Drafted SOPs and validation plans- Oversaw the NGS team to provide NGS service and bioinformatic support to research scientists- Established a tissue-specific ribosome-bound mRNA isolation method in Drosophila melanogaster- Identified dietary restriction-induced tissue-specific translation regulation- Uncovered the translation mechanism of mouse L1 retrotransposon- Identified L1 mRNA-binding cellular modulators of retrotransposition- Published over 20 peer-reviewed articles

Patrick L.'s Current Company Details
Johnson & Johnson Innovative Medicine

Johnson & Johnson Innovative Medicine

View
Associate Director of Nucleic Acid Technologies
California, United States
Website:
jnj.com
Employees:
100890
Patrick L. Work Experience Details
  • Johnson & Johnson Innovative Medicine
    Associate Director Of Nucleic Acid Technologies
    Johnson & Johnson Innovative Medicine
    California, United States
  • Johnson & Johnson Innovative Medicine
    Associate Director Of Nucleic Acid Technologies
    Johnson & Johnson Innovative Medicine May 2022 - Present
  • Sangamo Therapeutics, Inc.
    Associate Director Of Gene Specificity
    Sangamo Therapeutics, Inc. Jul 2020 - May 2022
    Brisbane, California, Us
  • Invitae
    Senior Scientist
    Invitae May 2017 - Jul 2020
    San Francisco, Ca, Us
  • Sangamo Therapeutics, Inc.
    Scientist Iii
    Sangamo Therapeutics, Inc. May 2013 - May 2017
    Brisbane, California, Us
    Performed and supervised NGS run and sequencing data analysis in the context of gene editing.Implemented and executed off-target studies of various gene editing nucleases.
  • Buck Institute
    Postdoctoral Research Scientist
    Buck Institute Jan 2008 - May 2013
    Developed technology in Drosophila to isolate tissue-specific ribosome-bound mRNA by immunoprecipitationPerformed and analyzed gene expression studies using RNAseq, microarray and high throughput qRT-PCRGenerated polysome profiles to monitor mRNA translation statusCreated overexpression plasmids using molecular cloning techniquesWrote basic scripts in R to analyze data and generate figures for publication
  • University Of Colorado Anschutz Medical Campus
    Phd Student
    University Of Colorado Anschutz Medical Campus Feb 2001 - Dec 2007
    Us
  • University Of Colorado Anschutz Medical Campus
    Graduate Research Scientist
    University Of Colorado Anschutz Medical Campus Feb 2001 - Dec 2007
    Us
    Identified and characterized Internal Ribosome Entry Sites (IRES) using luciferase reporter assayPerformed siRNA experiments and verified efficiency by Western blotCultured mammalian cells and performed cell-based assaysCreated reporter constructs using standard molecular cloning techniquesIsolated sequence-specific RNA binding proteinProbed for RNA secondary structureIdentified target sequence of RNA binding proteinMeasured affinity of an RNA binding proteinPurified overexpressed proteins from baculovirus infected cells

Patrick L. Education Details

  • University Of Colorado Anschutz Medical Campus
    University Of Colorado Anschutz Medical Campus
    Human Medical Genetics
  • Texas A&M University
    Texas A&M University
    Genetics

Frequently Asked Questions about Patrick L.

What company does Patrick L. work for?

Patrick L. works for Johnson & Johnson Innovative Medicine

What is Patrick L.'s role at the current company?

Patrick L.'s current role is Associate Director of Nucleic Acid Technologies.

What schools did Patrick L. attend?

Patrick L. attended University Of Colorado Anschutz Medical Campus, Texas A&m University.

Who are Patrick L.'s colleagues?

Patrick L.'s colleagues are Pascual Arizmendi, Andy Symes, Arvi D., Jeff Lol, Mina Yanny, Aleksandr Pelts, Lana Kandalaft.

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