Patrick Wanningen Email & Phone Number

Leiden

Working at the Janssen Vaccines and Prevention Institute, a Johnson & Johnson company, as an Associate Scientist at the department Subunit Vaccine Design (SUVD). Designing and executing new Universal Influenza (Flu) protein/DNA vaccine strategies and producing Adenoviral vectors.Research Vaccine development towards Infectious Diseases using cloning-, DNA molecular-, protein chemical-, virological- and cell culture techniques.Active member in the board of the Staff Association and triathlon organizationKeywords: Cell biology, Molecular Biology, Virology, Protein productions, Protein detection, Protein quantitation- and qualification and Database management.Working area's:- Influenza Virus (Flu)* cloning / miniHA / protein expression levels optimization (eg. other cell types, transfection by electroporation) / library screens / Retroviral vectors / Differential Scanning Fluorimetry- Adeno virus Adenoviral vector design, production & purification (DSP)* core field

Leiden

Working at the Crucell Vaccine Institute (CVI), a Johnson & Johnson company, as an Associate Scientist at the department Immunology and Proof of Concept (IPOC). Worked on two projects; designing and executing new DNA vaccine and antibody related strategies and experiments against (emerging) Flu and PSMA research.Research Vaccine development towards Infectious Diseases using cloning-, DNA molecular-, protein chemical-, virological- and cell culture techniques.Active member in the board of the Staff Association and triathlon organizationKeywords: Cell biology, Molecular Biology, Virology, Protein productions, Protein detection, Protein quantitation- and qualification and Database management.Working area's:- Influenza Virus (Flu)* cloning / miniHA / Reverse Genetics / protein expression- Prostate Specific Membrane Antigen (PSMA) cloning/protein expression * core fieldWorking experience for Crucell abroad Leiden:- at Crucell Inc., LA Jolla, CA. (USA) for PSMA protein generation and development 2012

Leiden

Working at Crucell Holland B.V as Research Technician, and later Sr. Research Technician, at the Department Innovation & Discovery Leiden within team Vaccine Generation. Worked on various projects; designing and executing new DNA vaccine and antibody related strategies and experiments against emerging Flu, Rabies, RSV & MV. Active member in the board of the Staff Association and triathlon organizationKeywords: Cell biology (cell cultures, internal cell staining), Molecular Biology (cloning, qPCR) and Virology; virus productions, Reverse Genetics, virus quantitation/proliferation assays, viral protein detection and qualification assays (eg. ELISA, protein purification, WB, SDS-PAGE) and database management.Working area's:- Influenza Virus (Flu)* cloning/Reverse Genetics/viral protein expression- Respiratory Syncytial Virus (RSV) viral expression systems - Measles Virus (MV) MV rescue experiments- Rabies Virus antibody screening developmentKeywords: Cell biology (cell cultures, internal cell staining), Molecular Biology (cloning, qPCR) and Virology; virus productions, Reverse Genetics, virus quantitation/proliferation assays, viral protein detection and qualification assays (eg. ELISA, protein purification, WB, SDS-PAGE) and database management.Working experience for Crucell abroad Leiden:- at Crucell, Bern Switserland in case of Measles virus technology transfer in 2009-2010.

Working at the Dept. of Medical Microbiology, section Molecular Virology, laboratory as a research laboratory technician using molecular biological, protein chemical and tissue culture techniques for human papillomavirus (HPV) research.

Graduate student with practical graduation position at the Animal Science Group Wageningen University and Research Centre, doing research at antigenetic variations in foot- and mouth disease virus (FMDV) persistent infected cattle by real time PCR techniques.