Patsy Green, Ms Email and Phone Number

•Performed viral neutralization immunoassays for agent SARS-CoV-2 robotically, using human serum to measure antibody-dependent activity while in the high containment BSL-3 environment. The assay screens donated human serum for anti-body resistance across multiple age groups, immunocompromised beings, and dilutions for vaccine robustness. Assays completed contributed to clinical trials resulting in the FDA approval of subsequent SARS-CoV-2 “booster” vaccines.•Developed an operational sera… Show more •Performed viral neutralization immunoassays for agent SARS-CoV-2 robotically, using human serum to measure antibody-dependent activity while in the high containment BSL-3 environment. The assay screens donated human serum for anti-body resistance across multiple age groups, immunocompromised beings, and dilutions for vaccine robustness. Assays completed contributed to clinical trials resulting in the FDA approval of subsequent SARS-CoV-2 “booster” vaccines.•Developed an operational sera inventory system for over 1,000 individual samples that were used for screening in immunoassays across 4 different vaccine product groups. Designed procedures and trained a team of trained analysts who were responsible for processing, documenting, and inventorying all collected blood samples in an efficient manner. Involved communication between manager and other existing department groups for transferring of samples for testing across multiple vaccine programs, acting as a leader for a group of analysts tasked with maintaining accurate information, and ensuring clinical timelines were met. •Contributed to the development of the attenuated SARS-CoV-2 viral neutralization immunoassay for HCID which will allow for execution in the BSL-2 environment and therefore allow for restructuring of department projects by avoiding the new for a strict high containment setting. Acted as a trainer for colleague’s skill assessing in laboratory techniques and improved team redundancy by authoring training manuals, guides, and standard operating procedures (SOPs). •Delivered key clinical data to department leads by being a qualified data reviewer who ensured all documentation was in compliance with cGMP/GLP and all other regulatory agency requirements. Show less

•Directed the Millipore filter plate qualification for all developmental and clinical immunoassay groups on VRD site. Designed testing protocols, prepared necessary reagents, and completed all data analysis to conclude which lots were qualified for use and further purchase from external vendor. Newly designed procedures exposed previous methods to be redundant and once revised will reduce annual testing budget for consumables by 75%.•Led large scale inventory compilation project of over… Show more •Directed the Millipore filter plate qualification for all developmental and clinical immunoassay groups on VRD site. Designed testing protocols, prepared necessary reagents, and completed all data analysis to conclude which lots were qualified for use and further purchase from external vendor. Newly designed procedures exposed previous methods to be redundant and once revised will reduce annual testing budget for consumables by 75%.•Led large scale inventory compilation project of over 3,000 individual samples and designed an operational sera inventory system which allowed for completion of SBA developmental/clinical assays. Involved communication between manager and other existing department groups for transferring of samples, pooling of sister samples, and use to produce long term proficiency panels for high-throughput clinical testing.•Managed inventory logistics of key consumable reagent for entire department by maintaining accurate volume tracking of samples and was responsible for placing orders from external reagents group to keep up with testing requirements to meet key clinical timelines. •Performed Neisseria meningitidis serogroup B (MnB) serum bactericidal assays (SBA) manually and robotically using human complement to measure antibody-dependent, complement mediated bactericidal activity of human sera. Execution of the manual assay tests complement lots and effectuate various assays for reagent qualification and developmental purposes. Assays completed contributed to clinical trials resulting in the FDA approval of pentavalent Mn vaccine.•Spearheaded the analysis of Secondary Mn strain which involved conducting primary screen plate triage to determine which individual complement lots from donors are fit to be used in developmental assays. Designed secondary screening and small mix analysis pools of specified strain to yield large scale samples to be used in clinical testing trials. Show less

“Study of the novel Boehringer Ingelheim Corporation produced Monoclonal Antibodies for use as Protein based Therapeutics.”•Investigated newly produced mAB’s and their reaction to environmental stresses including temperature dependence, viscosity, surface activity, and protein aggregation.•Produced bulk rheology data for various sample concentrations of a the given mAB-I stock sample which helped provide insight to the bigger picture to BI of whether or not this sample would be feasible… Show more “Study of the novel Boehringer Ingelheim Corporation produced Monoclonal Antibodies for use as Protein based Therapeutics.”•Investigated newly produced mAB’s and their reaction to environmental stresses including temperature dependence, viscosity, surface activity, and protein aggregation.•Produced bulk rheology data for various sample concentrations of a the given mAB-I stock sample which helped provide insight to the bigger picture to BI of whether or not this sample would be feasible for mass production.•Employed the newest technology of Rheo-Raman spectroscopy to track both physical and chemical changes of the sample throughout changing environments such as temperature. Show less

“Multiscale Modeling of the Oligomeric Protein Processes of Dynamin”•Used modern computing technology to simulate protein function specifically of Dynamin a GTPase responsible for endocytosis in the eukaryotic cell. Named as contributor in publication (see information below) highlighting the importance of this work to be able to help those that suffer from Alzheimer's and Parkinson's disease. •Led initiative for building tetramer backbone as well as stipulating environmental conditions… Show more “Multiscale Modeling of the Oligomeric Protein Processes of Dynamin”•Used modern computing technology to simulate protein function specifically of Dynamin a GTPase responsible for endocytosis in the eukaryotic cell. Named as contributor in publication (see information below) highlighting the importance of this work to be able to help those that suffer from Alzheimer's and Parkinson's disease. •Led initiative for building tetramer backbone as well as stipulating environmental conditions to track folding pathway of protein within the body. Completing study milestones allowed for funding to be secured for both summer research programs and the following academic year.•Employed coding platforms such as bash shell and python to create scripted notebooks for molecular simulation modelling. Show less

•Executed laboratory tasks and experiments under the guide of an experienced senior professor and acted as lead trainer to all undergraduate researchers.•Tutored chemistry of all undergraduate levels in the university help center and had the highest number of repeat sessions for students out of all other graduate assistants.•Managed sample preparation, laboratory equipment maintenance, and assignment grading for various undergraduate chemistry courses as a TA including quantitative… Show more •Executed laboratory tasks and experiments under the guide of an experienced senior professor and acted as lead trainer to all undergraduate researchers.•Tutored chemistry of all undergraduate levels in the university help center and had the highest number of repeat sessions for students out of all other graduate assistants.•Managed sample preparation, laboratory equipment maintenance, and assignment grading for various undergraduate chemistry courses as a TA including quantitative analysis, organic chemistry, and physical chemistry. As a second-year student was responsible for designing grading rubrics for laboratory courses for entire department and assigning sample preparation roles to other TA’s. Show less

•Managed a team of 5+ employees to complete tasks such as entering prescriptions, counting medications, and contacting insurance companies as well as doctors’ offices.•Dual responsibility of working under a pharmacist and aiding in the needs of 100’s of patients daily.•Solved critical problems via teamwork in order to please customers and satisfy their health needs.

“Improving the Performance of Granulated Activated Carbon to Remove Cr (VI) with Ascorbic Acid Ketal”•Examined the Chromium (VI), a byproduct of improper factory run off techniques as well as other industries such as leather tanning and textile production, reduction capabilities of an ascorbic acid derivative absorbed onto granulated activated carbon.•Determined the optimum experimental procedure for the production of GAC-Ketal compound which allows for drinking water to be treated to a… Show more “Improving the Performance of Granulated Activated Carbon to Remove Cr (VI) with Ascorbic Acid Ketal”•Examined the Chromium (VI), a byproduct of improper factory run off techniques as well as other industries such as leather tanning and textile production, reduction capabilities of an ascorbic acid derivative absorbed onto granulated activated carbon.•Determined the optimum experimental procedure for the production of GAC-Ketal compound which allows for drinking water to be treated to a safe and consumable concentration using a widely available and cost-effective reagent. •Analyzed the varying kinetic effects of chromium reduction reactions based various on stock solution concentrations using modern wet chemistry techniques. •Evaluated ways to recycle the reducing GAC-AAK complex.•Presented the ideas and findings of this project in an organized way to a body of about twenty other scientists. The work was then published in an annual research publication tilted the “Manhattan Scientist”. Show less

Advanced computer software including Spartan ‘14 and Maple were employed to not only build chemical structures, but also calculate specific variables of the compounds when placed in varying environments. This research project specifically focused on the degree to which energies of formation and dipole moments were affected when halogens of different electronegativies were substituted onto the compound.