Paul Hook Email & Phone Number

Baltimore, Md, Us

Baltimore, Maryland, United States

Postdoctoral fellow at Johns Hopkins University in the Department of Biomedical Engineering in Dr. Winston Timp's lab. Working on developing and applying genomics methods using Nanopore sequencing. Focus includes:- Developing and optimizing genomics approaches for measuring protein-DNA interactions using long-read sequencing including labeling protein-DNA binding sites with 6-methyladenine (6mA) and adapting CUT&RUN for nanopore sequencing- Generated long-read open chromatin data with 5-methylcytosine (5mC) GpC labeling and analyzed DNA modifications from short-read and long-read sequencing as part of the Telomere-to-Telomere Consortium- Planned experiments, generated data, and managed collaborations for a variety of projects including developing methods to store data in DNA and developing software to analyze nanopore signal data

Baltimore, Maryland

PI: Andrew S. McCallion- Designed, performed, and analyzed bulk RNA-seq and single-cell RNA-seq experiments on mouse dopaminergic neurons and established a scoring paradigm for prioritizing genes from Parkinson disease GWAS loci- Analyzed mouse chromatin data in order to identify cell populations relevant to neurological disease risk and to prioritize disease-relevant variation in disease-associated loci- Performed transgenic zebrafish assays in order to functionally validate putative enhancersfound within deletions associated with autism

Baltimore, Md

PI: Andrew S. McCallion- Explored the functional consequences of disrupting genes in zebrafish including effects onsomitogenesis and heart development- Developed and implemented the use of Cas9 nuclease genome editing in zebrafish andhuman cell culture in the lab- Managed the laboratory including being responsible for all ordering and working tomaintain safety and compliance- Trained lab members in relevant laboratory techniques and protocols

Sequim, Wa

PI: Michael Huesemann- Explored how temperature affected algal growth and algal lipid composition for the DOE’sNational Alliance for Advanced Biofuels and Bio-products team- Built and established the use of an algal culture thermal gradient incubator

University Park, Pa

Advisor: Joseph T. Keiser- Adapted and developed experiments focused on exploring the biochemical components ofpeanuts for an undergraduate laboratory class- Assisted in designing and building demonstrations for undergraduate chemistry lectures

Doctor Of Philosophy (Ph.D.), Human/Medical Genetics

Bachelor Of Science (B.S.), Biochemistry And Molecular Biology

Activities and Societies: Nittany NationMajored in Biochemistry and Molecular Biology