• Lead group in charge of producing vectors for R&D team, evaluating novel capsids for manufacturability, and screening new AAV production technologies / process improvements.• Co-developed, optimized, and iterated on novel plasmid designs to improve AAV yield and packaging.

• Evaluate novel technologies / materials for use in AAV production and purification• Run upstream production, downstream purification and some analytics depending on experiment requirements.• Act as SME for Vector Core operations• Run Vector Core and Process Development experiments as priorities / manpower necessitate.

• Develop platform process for downstream purification of various novel AAV vectors.• Run purification of multiple AAV vectors weekly.• Be trained on / occasionally perform cell culture, upstream production and analytical techniques.• Create SOPs and systems to document production processes.• Evaluate alternative purification strategies / materials / improvements.

• Operate various downstream manufacturing processes including tangential flow filtration, cation exchange chromatography, centrifugation, buffer preparation and sterile fill. Perform Upstream and Fill Finish operations as necessary.• Train new manufacturing personnel on downstream operations and buffer preparation.• Review documents both on and off the floor to ensure that documentation is being filled out appropriately and that manufacturing processes adhere to GMP requirements.• Validated a complete overhaul of step yield calculation procedure.• Revamped production schedule to allow for doubling of downstream throughput while ensuring adequate equipment and manpower was available to process multiple lots simultaneously.• Represented site on a cross functional team which completely reformatted and rewrote all purification batch records and SOPs.• Participated in FDA, EMA and PMDA audits.• Travelled to other manufacturing, contract manufacturing and process development sites to oversee and provide experience and insight on manufacturing operations.• Part of plant startup team to bring newly constructed state of the art gene therapy manufacturing site online.

- Teach students K-12 math after school.- Evaluate and document student progress.- Manage multiple students simultaneously working on multiple lessons.

- Worked with a varied team to see a project from experimental conception and development to finalreport writing.- Advised on experimental design of MRE burn emissions testing in order to develop a method thatwas feasible with the available materials, achievable within the deadline, and not tedious.- Set up various emission testing devices, collected data, changed many filters and stored old filtersfor transport.- Observed chemical analysis of collected emissions.- Performed analysis of various samples on GC-MS to determine origin of unexplained readings.