Project: Establishment of an assay to screen panels of antigen-specific antibodies for functional activity, supported by assay development• Responsible for high throughput identification of functionally active antibodies from panels of antigen-specific antibodies• Detailed characterization of antibody potencies and activities by using customized cell-based functional screening assays including ELISA, cell viability and reporter gene assays • Optimized assays to reduce reagent costs while maintaining detection sensitivity

Project: Pan and Phospho-specific Antibody Purification and Characterization. • Prepared affinity columns, and eluted rabbit sera for antibody purification and subsequent protein analysis• Characterized antibodies by running SDS-PAGE and western blots with various prepared cell and tissue lysates. Image acquisition and analysis on Bio-Rad Quantity One software.• Performed cell culture to maintain, treat and harvest cell lines required for antibody testing• Created and updated SOPs to facilitate ease of training for future employees