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Gonçalo C. Pereira Email & Phone Number
Scientist I
at
Astellas Engineered Small Molecules U.K. Ltd.
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✓ Verified August 2026
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Current company
Role
Scientist I
Location
Cambridge, England, United Kingdom
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Who is Gonçalo C. Pereira? Overview
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Gonçalo C. Pereira is listed as Scientist I at Astellas Engineered Small Molecules U.K. Ltd., a with 21 employees, based in Cambridge, England, United Kingdom. AeroLeads shows a matched LinkedIn profile for Gonçalo C. Pereira.
Gonçalo C. Pereira previously worked as Senior Project Scientist at Nanna Therapeutics and Research Associate at Mrc Mitochondrial Biology Unit. Gonçalo C. Pereira holds Phd from Universidade De Coimbra / University Of Coimbra.
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Astellas Engineered Small Molecules U.K. Ltd.
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Profile bio
About Gonçalo C. Pereira
I am an experienced biochemist interested in the role of mitochondria in health & disease. I am experienced in using biochemical techniques to study metabolism in vitro & in vivo along with live cell imaging, molecular biology and protein purification methods. I am a team player involved in the training and management of small teams comprising technicians & students. I am recognised as an experienced user in biostatistics, particularly in non-linear regression and data visualisation.
Listed skills include Bioenergetics, Mitochondria, Cardiovascular, Ischemia/Reperfusion, and 59 others.
Current workplace
Gonçalo C. Pereira's current company
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Astellas Engineered Small Molecules U.K. Ltd.
10 roles
Gonçalo C. Pereira work experience
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Scientist I
Scientist I
Senior Project Scientist
Nov 2022 - Apr 2024
Research Associate
Sep 2019 - Nov 2022
Research Associate
-- Prof. Ian Collinson Lab --This was a Wellcome Trust project for which I recommended a new technology to develop a novel biochemical assay. We developed a real-time luminescent assay based on the NanoBiT technology to monitor protein translocation in mitochondria. This has successfully reduced acquisition time from 2 days to minutes, providing high kinetic detail. I then moved to study the contribution of Δψ and ΔpH on protein import of native substrates using this assay. During my time in the lab I managed and supervised a PhD project for optimising the assay in mammalian cells.- yeast & bacteria culture - applikon bioreactor (30L) - protein expression (yeast & bacteria) - in vitro translation systems - protein purification - AKTA systems - molecular cloning (restriction enzymes, Gibson assembly) - protein & vector design - DNA extraction & purification - plasmid PCR - site-directed mutagenesis - Nanoluc - lentivirus production - lipofectamine transfection - enzyme kinetics (assay & modelling) - Python
May 2016 - Sep 2019
Research Associate
-- Prof. Michael Duchen and Prof. Gyorgy Szabadkai Lab --Here I was working jointly with GSK Neuroscience R&D (Richard Rutter) to confirm efficacy of pre-clinical drug candidates in inhibiting the mitochondrial permeability transition. I was responsible for development & optimisation of phenotypic assays, where I recommended two novel approaches to increase robustness. Partnering with big pharma helped me to develop time management & prioritising skills in order to regular produce and present data reports, discuss strategies and project directions.- mammalian cell culture - primary cell culture (cardiomyocytes & hepatocytes) - high-content screening - ImageXpress - MetaXpress - high-throughput cellular screening (FLIPR) - confocal microscopy - lipofection - flash-type luciferase - oxygraph - high-resolution respirometry - ImageJ - mitochondrial isolation - plate reader
May 2014 - Apr 2016
Research Assistant
-- Prof. Andrew Halestrap Lab --In this BHF project I was responsible for characterizing mitochondrial morphology and mitochondrial contact sites by EM upon ischemic injury and correlating it with ROS production & mPTP opening. I jointly lead the collaboration with the EM facility in order to solve practical issues and discuss results. I also taught myself how to use EM imaging process algorithms to accelerate our pipeline and meet deadlines.- animal handling - heart perfusion - perfusion protocols - mitochondria isolation - oxygraph - mitochondrial volume analysis - enzymatic activities - western blot - electron microscopy - light scattering - image processing
Nov 2012 - Dec 2013
Phd Student
-- Paulo Oliveira, PhD Lab --This project consisted of a multi-organ study of doxorubicin cardiotoxicity in a rat model, with special focus on mitochondrial dysfunction. Working with a technician I was responsible for daily handling and drug administration in animals under a sub-chronic protocol. This required me to develop planning and prioritising skills. I used a number of biochemical assay and applied concepts from control flux analysis to study the differential response of doxorubicin.-animal handling - animal experimentation - mitochondrial physiology - western blot - qPCR - mammalian cell culture - enzymatic activity on small high-throughput scale - mRNA extraction (tissues) - in vitro toxicity assays - tissue culture - cell assays - growth assays
Jan 2008 - Jun 2012
Internship
Scientific training for fulfilment of degree in biochemistry. Project consisted in studying the mechanism of action of the natural alkaloid berberine in vitro using two distinct toxicological models: isolated mitochondria (hepatic) and a cell line.- mitochondria isolation - mitochondria physiology - HPLC - mammalian cell culture - in vitro toxicity assays - fluorescence microscopy - toxicology profiling
Sep 2006 - Jul 2007
2 education records
Gonçalo C. Pereira education
FAQ
Frequently asked questions about Gonçalo C. Pereira
Quick answers generated from the profile data available on this page.
What company does Gonçalo C. Pereira work for?
Gonçalo C. Pereira works for Astellas Engineered Small Molecules U.K. Ltd..
What is Gonçalo C. Pereira's role at Astellas Engineered Small Molecules U.K. Ltd.?
Gonçalo C. Pereira is listed as Scientist I at Astellas Engineered Small Molecules U.K. Ltd..
Where is Gonçalo C. Pereira based?
Gonçalo C. Pereira is based in Cambridge, England, United Kingdom while working with Astellas Engineered Small Molecules U.K. Ltd..
What companies has Gonçalo C. Pereira worked for?
Gonçalo C. Pereira has worked for Astellas Engineered Small Molecules U.K. Ltd., Nanna Therapeutics, Mrc Mitochondrial Biology Unit, University Of Bristol, and Ucl.
How can I contact Gonçalo C. Pereira?
You can use AeroLeads to view verified contact signals for Gonçalo C. Pereira at Astellas Engineered Small Molecules U.K. Ltd., including work email, phone, and LinkedIn data when available.
What schools did Gonçalo C. Pereira attend?
Gonçalo C. Pereira holds Phd from Universidade De Coimbra / University Of Coimbra.
What skills is Gonçalo C. Pereira known for?
Gonçalo C. Pereira is listed with skills including Bioenergetics, Mitochondria, Cardiovascular, Ischemia/Reperfusion, Cell Biology, Biochemistry, Science, and In Vivo.
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