Curtis Perriotte-Olson Email & Phone Number

Papillion, Ne, Us

Smithfield, Virginia, Us

Smithfield, Virginia, Us

Smithfield, Virginia, Us

Washington, Dc, Us

• Organized and analyzed patient biobank data to discover clinical biomarkers.• Assisted with precision-cut liver slice cultures in human donor tissue.

Omaha, Nebraska, Us

ADMINISTRATIVE DUTIES: • Maintained supply inventories, MSDS, biosecurity and biosafety compliance per regulations. • Performed general maintenance on laboratory equipment to ensure an efficient work environment. • Acted as purchasing manager for the lab. Established accounts and communicated with vendors.• Managed new personnel training and assisted with the onboarding process.AUTHORSHIP and LITERATURE: • Assisted with writing and editing of grant proposals, manuscripts, posters and PowerPoint presentations. • Implemented and shared modifications to standard procedures to ensure reproducibility of data, transparency and collaboration among researchers.SOFTWARE and STATISTICAL ANALYSIS: • Proficient with Microsoft Word, Excel, PowerPoint, Photoshop, PRISM GraphPad statistics, ExpeData, LabChart, ImageJ and more.

Omaha, Nebraska, Us

ANIMAL CARE: • Primary lab manager and breeder of genetically-modified mouse colonies.• Maintained ongoing records of animal usage in accordance with federal law.LIVE-ANIMAL EXPERIMENTS: • Performed various metabolic analyses and live imaging procedures.• Completed tail-vein blood collection, subcutaneous injection, intraperitoneal injection and glucose monitoring.NON-RECOVERY ANIMAL SURGERY: • Induced and harvested peritoneal macrophage cells.• Performed axillary blood collection, organ perfusion and tissue harvest.

Omaha, Nebraska, Us

BIOCHEMICAL ASSAYS: • Spectrophotometer or fluorometer-based assays for aldehyde dehydrogenase, alcohol dehydrogenase, plasma alcohol, insulin and others. • Ligand binding assays.HISTOLOGY: • Knowledgeable of tissue fixation, processing, embedding and paraffin sectioning using a microtome. • Experienced in biological stains and fluorescence imaging.CELL CULTURE: • Primary hepatocytes, and ex vivo liver, muscle and adipose tissue. • Cultured additional cell-lines from adipose, muscle, liver, pancreatic cancer and fibroblasts.OTHER EQUIPMENT and TECHNIQUES: • Centrifuge, balance/scale, pH meter, spectroscopy (UV/VIS), microscopy (near-UV & visible), ECL and Odyssey protein imaging, immunodiffusion, RT-PCR, quantitative Real-time PCR, western blot, electrophoresis, fast protein liquid chromatography and more.

Omaha, Nebraska, Us

Chadron, Ne, Us

• [𝗔𝘂𝗴. 𝟮𝟬𝟭𝟭 - 𝗠𝗮𝘆 𝟮𝟬𝟭𝟯; 𝗖𝗵𝗮𝗱𝗿𝗼𝗻 𝗦𝘁𝗮𝘁𝗲 𝗖𝗼𝗹𝗹𝗲𝗴𝗲]. Studied oral bacteria biofilm formation and the inhibitory effects of polyphenols by culturing, staining and performing spectrophotometer analysis on bacteria.• [𝗝𝘂𝗻𝗲 𝟮𝟬𝟭𝟮 - 𝗔𝘂𝗴. 𝟮𝟬𝟭𝟮; 𝗗𝗲𝘃𝗲𝗹𝗼𝗽𝗺𝗲𝗻𝘁𝗮𝗹 𝗡𝗲𝘂𝗿𝗼𝘀𝗰𝗶𝗲𝗻𝗰𝗲, 𝗨𝗻𝗶𝘃𝗲𝗿𝘀𝗶𝘁𝘆 𝗼𝗳 𝗡𝗲𝗯𝗿𝗮𝘀𝗸𝗮 𝗠𝗲𝗱𝗶𝗰𝗮𝗹 𝗖𝗲𝗻𝘁𝗲𝗿]. Noted the effects of non-muscle myosin II on neural progenitor maintenance. I performed isolation of brain tissue in neonatal mice, cultured tissue and analyzed axon and dendrite growth. •[𝗠𝗮𝘆 𝟮𝟬𝟭𝟭 - 𝗔𝘂𝗴. 𝟮𝟬𝟭𝟭; 𝗩𝗔 𝗡𝗲𝗯𝗿𝗮𝘀𝗸𝗮-𝗪𝗲𝘀𝘁𝗲𝗿𝗻 𝗜𝗼𝘄𝗮 𝗛𝗲𝗮𝗹𝘁𝗵 𝗖𝗮𝗿𝗲 𝗦𝘆𝘀𝘁𝗲𝗺]. Assisted in recording the effects of leptin replacement on food intake and weight regain in diet-induced obese rats by helping with animal handling, food and weight measurement and immunohistochemistry of brain tissue.

Full-time summer employment. Primary responsibility of grounds maintenance (e.g. mowing and spraying). Assisted with other maintenance and animal care.

Bachelor Of Science - Bs, Biology - Molecular Biology Focus

Education record

Education record

Dry & Semi-Dry Sausage Short Course