Peter Gee Email & Phone Number

Kyoto, Jp

Asia Pacific

Kyoto, Japan

• Developed novel CRISPR-Cas9 ribonucleoprotein delivery system for in vivo application (patent pending)• Delivered CRISPR-Cas9 by plasmid or RNPs into human iPS cells by lipofection and electroporation using multiple platforms (Amaxanucleofector, NEPA21, and MaxCyte)• Genome edited Duchenne muscular dystrophy patient iPS cells for correcting disease phenotype• Performed ssODN knockin in iPS cells, achieving >60% efficiency• Differentiated human iPS cells into skeletal muscle… Show more • Developed novel CRISPR-Cas9 ribonucleoprotein delivery system for in vivo application (patent pending)• Delivered CRISPR-Cas9 by plasmid or RNPs into human iPS cells by lipofection and electroporation using multiple platforms (Amaxanucleofector, NEPA21, and MaxCyte)• Genome edited Duchenne muscular dystrophy patient iPS cells for correcting disease phenotype• Performed ssODN knockin in iPS cells, achieving >60% efficiency• Differentiated human iPS cells into skeletal muscle cells and cortical neurons• Generated various fusion DNA constructs of SpCas9 and fluorescent proteins for cellular tracking• Compared activity of ortholog CRISPR nucleases (Cpf1, SaCas9, StCas9, NmCas9)• Purified S. pyogenes Cas9 protein from e. coli for delivery into cells• Established stable cell lines by PiggyBac transposon vector system• Produced lentiviruses Show less

Kyoto, Japan

• Enhanced global visibility of the institute • Generated institute materials: 2 brochures, 4 pamphlets, 14 posters, and 3 newsletters • Engaged in public outreach events (scientific cafes)• Wrote 12 write press releases on scientific findings to reach a global lay audience through Eureka Alert

Palo Alto, California

• Screened small molecules weekly in 96-well biochemical assays to build structure activity relationship profiles• Worked autonomously and reported results weekly to project teams • Purified viral RNA polymerases for enzymatic characterization• Performed radioligand binding assays and radionucleotide-incorporation assays (32P, 33P, and 3H)Wrote DNA and RNA polymerase toxicity reports for IND filings with the FDA

Phd, Virology

Molecular Biology And Biochemistry