Peter Molek Email & Phone Number

Slovenia

Mengeš, Slovenia

Mengeš, Slovenia

Senior Scientist, DP Laboratory Process Development at Novartis Biologics Technical Development and Manufacturing

Ljubljana, Slovenia

- Development of an innovative and universal assay platform for detection of structurally diverse erythropoietin mimetics in biological specimens (international scientific project funded by WADA) - Design and development of novel fluorescence resonance energy transfer (FRET)-based assays and bacteriophage-based immunoassays for detection of novel performance enhancing drugs (doping substances) in biological specimens;- Chromatographic separation and analysis of therapeutic antibody… Show more - Development of an innovative and universal assay platform for detection of structurally diverse erythropoietin mimetics in biological specimens (international scientific project funded by WADA) - Design and development of novel fluorescence resonance energy transfer (FRET)-based assays and bacteriophage-based immunoassays for detection of novel performance enhancing drugs (doping substances) in biological specimens;- Chromatographic separation and analysis of therapeutic antibody aggregates - Studying peptide-protein interactions using microscale thermophoresis (MST)- Development of short peptide affinity ligands for antibody purification Show less

- Establishment (co-author of instructions books) and conducting lab courses in the subjects: - “Pharmaceutical biotechnology” (master’s study of Pharmacy), - “Cell biology and genetics” (undergraduate study of Cosmetology)- “Biotechnology in cosmetology” (undergraduate study of Cosmetology) and - “Drug biotechnology” (master’s study of Biotechnology at Biotechnical faculty);- Assisting and guiding individual undergraduate and PhD students during their laboratory research… Show more - Establishment (co-author of instructions books) and conducting lab courses in the subjects: - “Pharmaceutical biotechnology” (master’s study of Pharmacy), - “Cell biology and genetics” (undergraduate study of Cosmetology)- “Biotechnology in cosmetology” (undergraduate study of Cosmetology) and - “Drug biotechnology” (master’s study of Biotechnology at Biotechnical faculty);- Assisting and guiding individual undergraduate and PhD students during their laboratory research work (research projects, diploma, Master and PhD theses). Show less

- Development of leptin antagonists (single-chain variable fragments (scFvs) against leptin receptor):- Studying binding characteristics of scFvs by modified immunoassays (ELISA, FLISA);- Evaluation of bioactivity of isolated scFvs against human leptin receptor (LR) by mammalian cell proliferation assay and expression analysis using mammalian cells (qPCR, WB);- Recombinant protein expression (E. coli, mammalian cells) and purification (FPLC);- Design and preparation of… Show more - Development of leptin antagonists (single-chain variable fragments (scFvs) against leptin receptor):- Studying binding characteristics of scFvs by modified immunoassays (ELISA, FLISA);- Evaluation of bioactivity of isolated scFvs against human leptin receptor (LR) by mammalian cell proliferation assay and expression analysis using mammalian cells (qPCR, WB);- Recombinant protein expression (E. coli, mammalian cells) and purification (FPLC);- Design and preparation of alternative fluorescent probes based on filamentous phage particles- Optimization of phage display vectors: - design and preparation of an artificial helper phage for concomitant display of two distinct proteins on the same virion; - design and development of alternative gene delivery systems based on filamentous bacteriophages Show less

Doctor Of Philosophy (Phd), Biomedical Sciences, Pharmacy, Phd

Activities and Societies: - Screening phage display libraries of scFvs: selection of scFvs against human leptin receptor - evaluation.

Mpharm, Pharmacy