Peter Schweinsberg Email and Phone Number

Enriching food samples with GN media, bacterial lysis, PCR, gel electrophoresis, Aerobic Plate colony Counting, Enterobacteriaceae colony Counting, autoclaving, making GN media, washing bottles, taking/testing environmental swabs for contamination, streaking agar plates with bacteria, single and multi-channel pipetting, using LIMS

Extracting DNA from mouse tissue, PCR, molecular cloning

Molecular biology: Purification, digestion, and cloning of plasmid DNA; bacterial transformation through electroporation and chemical competency; microparticle bombardment of Caenorhabditis elegans; agarose and crystal violet gel electrophoresis; GTWY cloning, Gibson Assembly, PCR (Q5, Phusion, LongAMP, Herculase II polymerases), site-directed mutagenesis, reverse transcription of RNA, setting up reactions for Azenta to sequenceCell biology: some cell culture (transfection with FuGENE6; passaging CHO, HeLa, MDCK, COS, HEK cells); loading plates into an In Cell Analyzer 6000; making competent cellsProtein Characterization: SDS Page, Western blotting; using a developerGeneral: Caenorhabditis elegans culture and maintenance; pouring NGM plates; making antibiotic plates and solutions (freezing solution, S Basal, M9 salts, LB media, 2XYT media); autoclaving; laboratory maintenance; fluorescence microscopy