Graduate Research Assistant
Current· Successfully designed novel chimeric proteins to treat metabolic diseases like diabetes, obesity, liver diseases with high efficiency and produced results 6 months faster than projected deadlines.· Proficient in proteins production, expression (BL21 DE3, plysS, Origami, Rosetta, etc.) and purification (automated FPLC, column-chromatography, affinity, ion exchange, SEC, etc.)· Proficient in biophysical assays, protein structures characterizations and protein-ligands interactions (SDS-PAGE, ITC, DSC, fluorescence spectroscopy, CD, UV-Vis, ANS binding, etc.) · Familiar in cell-based assays, bacterial and mammalian cell culture, cell differentiation, bioreactor production· Experiences with routine data processing, analysis, and visualization.· Familiar with designing, executing and analyzing biochemical methodologies for new instrumentation and technologies to save costs and improved protein purification results by 30%.· Collaborative, self-directed, highly motivated, enthusiastic about learning new technologies, research and innovation in molecular biology and protein biochemistry.· Excellent communicator (oral and written), independent worker and critical thinker with strong foundational knowledge about protein sciences.· Trained new undergraduates and graduate students with current established protocols and instruments· Assisted current undergraduates and graduate students with research works· Wrote and awarded grant applications for undergraduate and graduate research projects in university and national levels· Managed and maintained laboratory instruments and chemicals as well as coordinated with appropriate personnel for fixing and maintaining equipment· Completed audits, purchase orders, invoices, and other general bookkeeping duties, as well as stored chemicals and equipment upon receipt to ensure the proper storage of purchases
