A. Pierce Von Buttlar Email & Phone Number

Portland, Oregon Metropolitan Area

Responsibilities:▪ Plan and execute in-house upstream Process Development (PD) studies to optimize viral vector production and scale-up in flatware (T flasks, Hyper Stacks) and bioreactors (fixed-bed, stirred tank).▪ Perform in-process analytical testing▪ Drive PD activities (i.e. process investigation, definition, optimization, robustness) to improve understanding of current virus production processes and develop next-generation manufacturing processes for advanced clinical trials.▪ Oversee and lead technology transfer and execution of feasibility and optimization studies at external partners.▪ Transfer processes to manufacturing facilities and drafting/review of batch records and technical documents to help enable execution in GMP environment.▪ Author publications and documentation to support regulatory submissions.▪ Serve as internal domain expert working closely with cross functional teams and external partners (CDMOs, Suppliers, etc.).Achievements:Adapted flatware production process for use with microcarrier technology in stirred tank reactors.Optimized an upstream process using microcarriers to massively increase production output to meet projected vaccine demand.Successfully transferred process to CDMOs and managed further optimization.

Vancouver, Washington, United States

Responsibilities: Working with high throughput screening teams to screen thousands of candidate bacterial strains and libraries to with the goal of discovering an optimal producer of target biologic. Worked with strain construction, NGS, ELISA, and flow cytometry teams to find the ideal strain to produce the client’s target protein.Achievements:▪ Established ‘BioServices’ department with the goal of improving and streamlining glycerol stocking process to generate single use aliquots and implement rigorous quality control and electronic lab notebook documentation including barcode scanning to track inventory and results metrics in newly implemented LIMS systems.▪ Collaborated with drug discovery department to create induced and uninduced glycerol stock libraries.▪ Worked with Upstream, Downstream, analytical and program management departments to create a traceable combined sample inventory LIMS and freezer system.

Harmans, Maryland, United States

Responsibilities: Managed several client projects involving large-scale manufacture of gene therapy and Vaccine biologics. Lead teams performing upstream biologics production using adherent and suspension cell lines in grade D-A clean rooms. Lead processing beginning with aseptic BSC passaging, cell banking, and seed train expansion with suspension and adherent flasks through hyperstacks and WAVE reactors. Also lead teams in Bioreactor operations; inoculation, transfection/infection, monitoring, through biologics harvest and clarification. Worked with a variety of bioreactors; WAVE, Sartorious twin, iCELLis, and Xcellerex. Also performed filtration, clarification and purification using POD depth filters, Pall Stax, and Opticap clarification. Upstream purview extended through TFF/KTF concentration and diafiltration using cassette and hollow fiber filters.Achievements:▪ Revised and wrote Batch Production Records and SOPs for upstream production. ▪ Trained 3 teams of manufacturing associates; each designated to separate clients using different methods of production for gene therapy and vaccine biologics. ▪ Began as Manufacturing Associate I (MAI) Promoted to MAII within first 6 months of employment, and to Team Lead (a.k.a. MAIV) within a year.▪ Established three manufacturing suites from the ground up. Built, trained, and lead teams in these suites for the manufacture of Astra Zeneca COVID19 vaccine.

Phoenix, Arizona Area

Responsibilities: Conducted project involving manufacture of microarrays for clinical applications. Executed every step of production line; incoming chip inspection, plasma cleaning of chips, drop shape analysis contact angle determination, surface functionalization, probe preparation and printing, blocking, post dispense imaging and array inspection, quality control assay and analysis, packaging and shipping. Collaborated with quality assurance department on time studies and risk management investigation. Worked with scientists to design and carry-out R&D experiments for product optimization. Collected data on functional assay fluorescence intensity trends and product yield to present at weekly progress meetings. Achievements:▪ Designed procedure improvements for production ramp-up, successfully doubled daily output▪ Implemented time study and cost analysis for automation proposal, assisted in automating aspects of production line▪ Diagnosed issues with incoming substrate and coordinated corrective revisions with supplier to reduce incoming scrap▪ Managed databases for inventory, oligo concentration, QC assay trends, scrap and failure rates

Austin, Texas Area

Responsibilities: Managed patient samples from accessioning through histological slide manufacturing in collaboration with pathology partners. Performed Quality Control duties; routinely checked patient information, and sample quality. Processed a variety of cytological specimens according to type and fidelity. Operated and maintained, Thinprep and Cellblock instruments. Recorded detailed metrics of lab process efficiency. Communicated with pathologists, submitting physicians, and Veracyte genetics branch to generate detailed reports and diagnoses. Completed detailed documentation and SOPs adhering to cGLP, cGMP, CLIA and CAP regulations. Achievements:▪ Streamlined procedures to increase production, processed over 5,000 specimens per month▪ Managed database of proficiency sample reports and lab utilization metrics▪ Interviewed potential employees and trained coworkers▪ Presented lab utilization data and safety committee instructional sessions

Austin, Texas Area

Responsibilities: Organized and performed assorted ELISA and immunological assays for clients on various sample types. Processed samples and calculated standard and dilution curves. Performed Quality Control duties; routinely audited reports, results and sample quality against control parameters. Recorded metrics, analyzed and reported spectrophotometric data. Created and inventoried reference ranges. Completed detailed documentation and SOPs on assays adhering to cGLP, cGMP, and CAP regulations. Achievements:▪ Streamlined procedures to increase production, tested over 20,000 samples per month▪ Interviewed and trained new team members, assisted in automating several job functions▪ Routinely troubleshot uncharacteristic results and QC failures

Seton Hospitals

Responsibilities: Tracked high volume of patient samples, entered results and reports quickly. Performed Quality Control duties; routinely audited patient information, reports, results and sample quality. Collaborated with microbiology, hematology, chemistry, serology, and urinalysis departments. Completed meticulous records on all patient testing, abided by HIPAA, CAP and CLIA guidelines.Achievements:▪ Monitored 4000 patient tests per month, delegated tasks, and trained new team members▪ Optimized patient care by eliminating erroneous test orders and monitoring specimen quality▪ Resolved billing issues between hospital/third-party labs and patients▪ Assisted physicians with testing parameters, specimen requirements, and result interpretation

Sacramento Ca

Responsibilities: Executed large scale histological processing of human and primate brain tissue. Performed sectioning and used a variety of staining procedures to create whole brain slide catalogues. Performed Quality Control duties; routinely audited, results, statistical analysis, sample quality, and examined slides to determine extent of cell activation with sampling grid software. Conducted data analysis and presented trends using statistical modeling in Excel. Coordinated with vendors to order lab supplies, and prepared lab for regulatory safety and compliance audits. Wrote and reviewed reports and protocols. Curated tissue case files and confidential patient records.Achievements:▪ Trained and managed 5 undergraduate interns in histology procedures, delegated tasks and checked for quality▪ Optimized histological processes and increased production by 400%▪ Performed literature reviews and planned experiments to optimize immunostaining▪ Completed slide libraries for the Amaral Lab and third-party labs, now featured in published papers

Davis, Ca

Responsibilities: Studied regulatory pathways in soil bacterium. Produced bacterial mutants to study gene functions involved in morphological development. Utilized PCR, RT-PCR, and various selection methods to build plasmids and test mutant strains. Trained in electroporation and transformation techniques to incorporate customized DNA into bacteria. Designed plasmids to delete specific genes using BLAST libraries, restriction digest and ligation reactions. Controlled for quality by of sufficient custom DNA concentration and purity with Nanodrop.Achievements:▪ Created 5 mutant strains, and wrote reports on potential gene function using mutant data

San Diego State University, San Diego, Ca

Responsibilities: Assisted with cardiovascular research by investigating cardiomyocyte mitochondrial fusion/fission. Conducted an independent project to procure and test Acai fruit products for Trypanosoma Cruzi parasite DNA. Produced plasmids for protein expression in bacteria and transfected Mouse embryonic fibroblasts, and evaluated proteins for cardioprotective properties. Used fluorescent microscopy to QC for plasmid incorporation.Achievements:▪ Reviewed literature and developed an assay to distinguish T.Cruzi DNA in a noisy sample background by using distinctive kinetoplast minicircle DNA primers▪ Created and purified 3 different proteins and evaluated MEFs for rate of mitochondrial fusion

Davis, Ca

Solidified my understanding of biology by teaching small groups of 7 students daily. Saw significant increase in grades. Learned public speaking, leadership, and teaching skills. I was trained in positive reinforcement, leading question, and response eliciting techniques. I was required to prepare a lesson and practice questions for each session. Gained communication, time management and interpersonal skills.

Greater San Diego Area

Collaborated with a group of interns, and we compiled contact databases, attended product presentations and evaluations, contacted and met with potential sponsors and members, and gave presentations regarding our progress in member acquisition and overall progress to a board the CONNECT Board of Directors.