Mission: Study the role of Rab6 protein in migration and gut homeostasis.Techniques used:-Mice dissection of small intestine, vibratome sectioning and immunofluorescence.-Cultivation of adherent cells and 3D intestinal organoids.-RNA extraction and Real-time qPCR (SYBR/Taqman).-Use of several microscopes: confocal, video, spinning disk.Scientific publication : The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epithelium. Simonin et al., Development, 2024.

Missions: Study the interactions between proteins and centromeric DNA.Techniques used: -Production and purification of recombinant proteins using affinity and size exclusion chromatography (Akta), followed by Western blot analysis.-Gibson assembly cloning.-Management of orders and team database.

Missions: Production and purification of antimicrobial peptides from bacteria (lasso peptides).Techniques used:-Directed mutagenesis followed by bacterial transformation. -Microbial culture and peptides purification by HPLC.

Missions: Production and purification of S-layer proteins from a hyperthermophilic archaea and management of the shared service. Techniques used:-Archaea culture, extraction and separation of proteins by exclusion chromatography followed by analysis using SDS-PAGE electrophoresis. -Order management and GLP training for new entrants.Scientific publication : Improved Isolation of SlaA and SlaB S-layer Proteins in Sulfolobus acidocaldarius. Simonin et al., Extremophiles, 2020.

Missions: Study of the role of the FcεRI IgE receptor on blood platelets in anaphylaxis.Techniques used:-Mouse genotyping by PCR.-Isolation of blood platelets by washing protocol.-Analysis by flow cytometry (Gallios), Western-blot and ELISA tests.

Missions: Study of the accumulation of F-specific RNA bacteriophages in dreissenes.Techniques used: -Bacterial culture.-RNA extraction and RT-qPCR.