Piroska Leonard Email & Phone Number

Villigen, Aargau, Switzerland

Paris, Île-De-France, France

Extention of the PhD contract- Worked in binome with another engineer, designing and managing their experiments

Paris, Île-De-France, France

- Piloted an interdisciplinary scientific research project, regarding the aggregation of proteins in neurodegenerative diseases. Participated actively in all steps of the project, from conception, experimental execution, to data analysis interpretation, article writing and publishing.- Developped a methodology to robustly reconstitute a condensed phase of the protein α-synuclein in human cells, which allowed unprecedented insight onto its potential aggregation pathways.- Acquired solid… Show more - Piloted an interdisciplinary scientific research project, regarding the aggregation of proteins in neurodegenerative diseases. Participated actively in all steps of the project, from conception, experimental execution, to data analysis interpretation, article writing and publishing.- Developped a methodology to robustly reconstitute a condensed phase of the protein α-synuclein in human cells, which allowed unprecedented insight onto its potential aggregation pathways.- Acquired solid skills in human cell culture maintenance and experimentation, transient DNA expression, microbiology, molecular cloning, immunostaining techniques and microscopy imaging (both quantitative and qualitative).- Published results in a peer-reviewedscientific journal: L Piroska et al. “α-Synuclein liquid condensates fuel fibrillar α-synuclein growth“ Science Advances, https://www.science.org/doi/10.1126/sciadv.adg5663- Communicated results to peers at the Research Consortium: “Quantitative approaches for lifesciences” (2022) and the Jacques Monod international conference: "Protein phase transitions in aging and age-related diseases: from atomic resolution to cellular solutions" Show less

Paris, Île-De-France, France

Assisted professors of Sorbonne Université, by teaching courses to Bachelor's degree students, in three different subjects:- Analytical Chemistry- Perfumery Chemistry - Thermodynamics

Paris, Ile-De-France, France

Contributed to a project aiming to engineering of artificial cellular Ribonucleoprotein (RNP) granules- Assisted a PhD student in the design and experimental validation of a protein scaffold that allows the controlled formation of artificial cellular organelles that recruit RNAs- Performed molecular clonings of DNA plasmids- Cultured, maintained and transfected mammalian cell cultures- Performed fluorescence microscopy on both live and fixed cells- Analyzed microscopy images… Show more Contributed to a project aiming to engineering of artificial cellular Ribonucleoprotein (RNP) granules- Assisted a PhD student in the design and experimental validation of a protein scaffold that allows the controlled formation of artificial cellular organelles that recruit RNAs- Performed molecular clonings of DNA plasmids- Cultured, maintained and transfected mammalian cell cultures- Performed fluorescence microscopy on both live and fixed cells- Analyzed microscopy images using ImageJ Show less

Paris, Ile-De-France, France

Was in charge of a project aiming to uncover the structure of a protein involved in tRNA modifications- Designed and optimized protocols for the expression and purification of the protein- Worked with the protein in anaerobic conditions (glovebox) to reconstitute its O2-sensitive Fe-S cluster.

Nice Area, France

Participated in a study run by the University of Nice, aiming to assess the professional success of its alumni, several years after graduation.- Programmed and performed calls to collect data for the study- Managed an anonymized database with the alumni's answers to the study

Nice, Provence-Alpes-Côte D'Azur, France

Contributed to the experimental part of a research project on melanoma, by synthesizing a therapeutically promising molecule.Contributed to the conception and synthesis of an analog library for the target molecule.

Nice Area, France

Contributed to a research project regarding the development of novel, eco-friendly epoxy resins.Assisted a PhD student in the experimental procedures related to the synthesis and purification of furane-based époxy resins.

Master'S Degree, Chemistry And Life Sciences

Pluridisciplinary program at the frontier between Chemistry and Biology

Bachelor'S Degree, Chemistry