Pablo Martinez-Acedo Email & Phone Number

Valladolid, Castilla And Leon, Spain

• Analytical Professional Services• Service support for Waters High Resolution Mass Spectrometry and UPLC systems, applications and software• Specialist support for Waters developed biopharmaceutical applications: Intact Mass Analysis of Proteins & Oligonucleotides, Monoclonal Antibody (mAb) Subunit Analysis, Released N-Glycan Analysis, Peptide Mapping/Multi-Attribute Monitoring (MAM) , etc.• Customer support and training• High quality customer experience

Barcelona, Catalonia, Spain

• EU_Clinical group• Service support for Waters LC/MS hardware, applications and software• Specialist support for Waters developed clinical applications. NewBorn Screening, Immunosuppressants, Vitamin D, Steroids, etc.• Frontline support for new Clinical System Solutions developments.• Clinical Operator Development Training and post-implementation on-site visits as to initiate the clinical application.• Assuring a high level of cGMP compliance through the Clinical Complaint Handling Process for Waters Clinical product offerings. Ensure complaint investigations are complete, thorough, and meet regulatory reporting requirements both domestic and international.• Ensure that quality system procedures and Health & Safety standards are adhered to at all times.• High quality customer experience

The Proteomics Core at Scripps Florida offers a wide range of mass spectrometry-based proteomics services to assist with protein characterization, identification and quantification. It is essential to examine the expression and action of proteins and other gene products during normal conditions as well as disease state. The core provides support to Scripps faculty and staff who focus on such questions. In particular, the scientists concentrate on developing and applying the techniques of mass spectrometry for discovery and quantitative proteomic experiments. Listed below are the available services provided by the proteomics facility:1. Proteomics: Preparation of protein samples (in-gel or in-solution proteolytic digestion) and nano-HPLC-MS/MS characterization of simple and complex protein mixtures including whole cell lysates. Mapping of posttranslational modifications. Sample enrichment strategies for posttranslational modification analysis. Redox proteomics approaches. IP-MS. Quantitative proteomics analysis of metabolic labeled (SILAC) samples. Identification of chemically isotope labeled proteomes using Tandem Mass Tags (TMT-6-plex) for quantitative proteomics analysis. Quantitative proteomics approaches through 18O enzymatic labeling. Perform database searches with multiple search engines (Sequest, Mascot, ProLuCID, X!Tandem, MaxQuant, MassMatrix). Data analysis tools include Scaffold Q+S, Census, Trans Proteomics Pipeline, Ingenuity.2. Biomolecular Mass Spectrometry: Top-down proteomics by liquid chromatography mass spectrometry (LC-MS) to determine the intact protein molecular weight and relative quantification. Antibody-drug conjugate analysis.3. HPLC Purification: Peptides or small proteins can be purified using reverse phase separation. Detection systems include UV, multi wavelength and fluorescence.4. Isothermal Titration Calorimetry approaches for thermodynamical studies.

Kate Carroll Lab

Improvement of tools and high-throughput proteomics workflows for the study of Cysteine oxidation (mainly Cysteine sulfenic acid) and its application to study physio-pathological processes of biological relevance

Jesús Vázquez Lab

Development of O18 labeling techniques and high-throughput peptide analysis by mass spectrometry applied to the molecular study of the thiol redoxome dynamic state.

Skin And Superficial Aspects - Stratum Corneum Laboratory, Clichy - France

Development and improvement of a workflow for the study of the heparanome, or proteins associated to glycoproteins type heparane sulfate, of the upper layers of the skin (Stratum Corneum) in order to purify a growth factor for tests in cell cultures or in vitro skin models.

Molecular Research On Antibiotics Laboratory, Inserm (Ufr 970)

Protein sequences alignment and Mycobacterium tuberculosis GyrA subunit 3D-modelling in order to elucidate its action mechanism

Immune Regulations And Development Laboratory

Study of cells passage from mother to foetus, in Mouse model, and its implication in vertical transmission of prion protein

Phd In Molecular Biology (Proteomics)

“Development of novel stable isotopic labeling techniques and peptide high-throughput analysis by mass spectrometry: application.

Master Of Science (M.Sc.), Biologie Et Pharmacologie Du Vieillissement

Bachelor In Sciences, Cell Biology And Physiology