Genome editing in various crop plants

CRISPR/CAS9-Genome editing and transgenic plant development in rice

To explore the role of LTR retrotransposons in plant genome evolution and epigenetic modifications under abiotic stress conditionsPlant transformation and tissue culture Arabidopsis floral dip method Moso bamboo-carbon nanotube (CNT) diffusion method Eggplant and tobacco Agrobacterium-mediated genetic transformation Wheat in-planta genetic transformationCloning and construction of vectorsFocused on the cloning and characterization of several RNAi constructs like, pMDC164, pMDC43, pBI121, pK7GWIWG2(I) vector (RNAi GATEWAY ready). pGADT7-Rec Vector, pGBKT7, pSPYNE-, pSPYCE

CRISPR analysis

Engaged in Molecular Biology works ilke PCR techniques (DNA & RNA extraction, cDNA synthesis, conventional PCR, and qRT-PCR). Southern and western blotting, yeast one and two-hybrid systems, in-situ hybridization, gene expression analysis through qRT-PCR, and expression analysis of siRNAs using Northern blotting.  Gateway cloning, restriction-based enzyme cloning and ligation, Transformation, Plasmid purification, and sequence analysis. Design, construction, and functional verification of new RNAi vectors based on molecular and genetic studies related to the projects