Prasad Devarshi, Phd Email & Phone Number

Los Angeles Metropolitan Area

Los Angeles, California, United States

Greater Los Angeles Area

Greater Los Angeles Area

• Planning and execution of various experiments with knockout animal models to study circadian rhythm and metabolism • Using chromatin immunoprecipitation assays to study metabolic regulation by clock genes

• Managed clinical study involving transcriptomic, epigenomic & metabolomic analysis in 30 human subjects• Performed bioinformatics analysis, pathway analysis, gene ontology analysis and interpretation of NGS data for 5 projects (including 2 collaborations)• Whole-genome next generation sequencing (NGS) analysis and interpretation of transcriptomics (RNA-sequencing) and epigenomics (DNA/MNase sequencing) data using various bioinformatics tools to identify differential gene expression and epigenetic changes in Linux using cluster computing and amazon web services• Dissertation: Effects of Acute Aerobic Exercise on Whole Genome Nucleosome Maps, Transcriptomics and Acylcarnitine Metabolomics in Skeletal Muscle of Lean Vs Overweight/Obese Men• Sample extraction for RNA-seq/MNase-seq and other molecular/biochemical experiments • Wrote and revised manuscripts (4 publications so far, including 3 first-author)• Took initiative to be the first lab member to learn RNA-seq data analysis• RNA-seq analysis (Transcriptomics)• MNase-seq analysis (Epigenomics)• Bioinformatics analysis tools: DAVID, KEGG, SAMtools, FASTQ Groomer, TopHat 2, Bowtie 2, CuffDiff, CummeRbund, UCSC Genome Browser, JMP Genomics and HOMER• NGS analysis in RedHat Linux environment and Ubuntu on Amazon Web Services• Metabolomics data analysis• Research areas: Type 2 diabetes, obesity, mitochondrial dysfunction, skeletal muscle lipid metabolism, epigenetic mechanisms, nucleo-mitochondrial crosstalk

• Recipient of the ‘Young Scientist Research Grant’ for the project entitled: ‘Evaluating Combinatorial Supplementation of Flaxseed Oil and Fish Oil with Statins and Hypoglycemic Drugs as a Novel and More Effective Anti-Diabetic Strategy’• Funding for above project: $8,800 in grant funding utilized (total funding: $28,000 for 3 years)• Led the drug and diet intervention study in diabetic animal model• qPCR protocol and data analysis, RNA extraction and other molecular and biochemical experiments• Interpreted and presented the data, submitted progress reports and managed the budget for the project• Research areas: Type 2 diabetes, diabetic dyslipidemia, hepatic lipid metabolism

• Participated in 7 collaborative projects involving qPCR studies with industry and other labs at IRSHA• Took initiative to be one of the first lab members to learn qPCR study protocol and analysis• Analyzed differential gene expression following interventions in animal models of various diseases• Helped write manuscripts (6 publications so far, including 1 first-author)• Performed molecular/biochemical experiments collaborative and other on-going projects in the lab• RNA extraction from cell culture, liver, kidney, synovial membrane, cDNA synthesis and western blot• Research areas: Hepatic and renal complications in diabetes, protein glycation, systemic inflammation

• Managed Master’s research project (Thesis tile: Nutrigenomics: Studying the Effect of Omega-3 Supplementation on Lipid Metabolism)• Carried out molecular and biochemical experiments for the diabetic animal model study and other projects• Research areas: Diabetic dyslipidemia, hepatic lipid metabolism

Doctor Of Philosophy (Ph.D.), Focus Area: Ngs Bioinformatics Analysis, Molecular Biology, Epigenetic Mechanisms & Lipid Metabolism, Gpa: 3.67

Dissertation title: Effects of Acute Aerobic Exercise on Whole Genome Nucleosome Maps, Transcriptomics and Acylcarnitine Metabolomics.

Master'S Degree, Pharmacy, Specialization: Pharmaceutical Biotechnology

Thesis: Nutrigenomics: Studying the Effect of Omega-3 Supplementation on Lipid Metabolism

Bachelor'S Degree, Pharmacy