Prasad Parchuri Email & Phone Number

Pullman, Wa, Us

United States

1. Unraveled the molecular mechanism underlying industrially important hydroxy fatty acids biosynthesis in oilseed crop Physaria fendleri by molecular and biochemical approaches.2. Successfully purified the lipase and acyltransferase proteins involved in novel triacylglycerol remodeling pathway in Physaria by recombinant expression and affinity-based protein purifications.3. Characterized the purified proteins by developing novel enzymatic assays.4. Collaborated with a team of plant molecular biologist to develop transgenic lines producing hydroxy fatty acids.5. Trained undergraduates and led technicians in protein purification and lipid characterization.

Bengaluru, Karnataka, India

1. Optimization and validation of new tests following CAP guidelines and lab policies. 2. Preparing NGS libraries for various panels and new protocols including ampliconsequencing libraries, whole genome libraries, whole exome libraries, target panellibraries etc.3. Troubleshooting, optimizing, validating and implementing new lab protocols based onNGS, Sanger sequencing and fragment analysis.4. Preparation of SOP , validation documents, worksheets and maintaining documentationsrequired as per the CAP criteria.

Mysore

1. Planned and executed the project entitled " Exploring lipid biosynthesis in the seeds of Buglossoides arvensis: A rich natural source of stearidonic acid.2. Used advanced next generation Omics such as Transcriptomics and lipidomics to understand stearidonic acid rich oil biosynthetic pathway in B. arvensis.3. Various genes involved in ω-3 fatty acids and oil biosynthesis were functionally characterized in yeast and Arabidopsis model system using multi-gene cloning approach.4. Used various high-end analytical techniques such as HPLC, GC-MS, LC-MS to characterize the seed lipids.5. Trained new lab members on various molecular biology techniques and provided support and mentoring.6. Documented the results and written annual reports and publications.

Mysuru Area, India

• Executed the DBT sponsored project entitled "Probiotics for antigen delivery.• Screening of probiotic bacteria for antimicrobial proteins.

Manhattan, Kansas

• Received extensive training in SCIEX 6500+ Mass Spectrometry instrument handling, data acquisition and processing.• Characterized the lipidome of developing seeds of Buglossoides arvensis to understand omega-3 fatty acids rich triacylglycerol biosynthesis.• Contributed to the LIPID MASS MAPS database project.

Kadapa

• Planned and executed the project entitled "Zinc –Microbe interactions".• Isolation and characterization of Zinc-resistant microbes.• Preparation of annual reports and publications.

• Training on "Overview of Biopharma Production and Engineering in Biozeen (Sponsored by DBT, Government of India)• Training in Animal cell culture, Microbial fermentation, Downstream processing. Trained in protein purification by chromatography techniques and characterization.

Doctor Of Philosophy - Phd, Biological Science, Cgpa:9.5

Fulbright Nehru Doctoral Research Fellow, Plant Sciences; Lipidomics

Master Of Science - Ms, Biotechnology, 79%

B.Sc, Biotechnology, 83%

Bachelor of Science in Biotechnology

Honour Of Diploma, Computer Application

HDCA

Intermediate, Botany, Zoology, Physics, Chemistry

Intermediate

Education record

Secondary School Certificate