Preethi Cherian Email & Phone Number

- Experience in Molecular editing tools - CRISPR Cas9 system.- Tranfections involving miRNA, siRNA, miRNA MIMICS.- Extraction of DNA/RNA/Protein from PBMCs/Adipose tissue samples or cell lines.- cDNA synthesis and Polymerase Chain Reaction for gene expression analysis. - Chromatin Immunoprecipitation (ChIP) Assay.- Transformation & Cloning.- Preparing Maxi preps & Minipreps of the transformed vectors & appropriate storage.- Cell culture- Transfection and… Show more - Experience in Molecular editing tools - CRISPR Cas9 system.- Tranfections involving miRNA, siRNA, miRNA MIMICS.- Extraction of DNA/RNA/Protein from PBMCs/Adipose tissue samples or cell lines.- cDNA synthesis and Polymerase Chain Reaction for gene expression analysis. - Chromatin Immunoprecipitation (ChIP) Assay.- Transformation & Cloning.- Preparing Maxi preps & Minipreps of the transformed vectors & appropriate storage.- Cell culture- Transfection and treatment of cell lines.- ELISA.- Blood processing for PBMC extraction Show less

Molecular Biology:• PCR, RT-PCR, Nested-PCR, Semi-Nested PCR, Multiplex PCR , Real-time PCR Techniques.• Extraction of DNA from Thick and Thin smears(on slides). DNA extraction from whole blood/tissues samples etc.• Poly-acrylamide Gel Electrophoresis (PAGE), Agarose Gel Electrophoresis( AGE).• RAPD.• Gene Sequencing and analysis of the sequence using BLAST. ( familiar with Bioinformatics)• Cloning techniques . Plasmid extraction.• Cell… Show more Molecular Biology:• PCR, RT-PCR, Nested-PCR, Semi-Nested PCR, Multiplex PCR , Real-time PCR Techniques.• Extraction of DNA from Thick and Thin smears(on slides). DNA extraction from whole blood/tissues samples etc.• Poly-acrylamide Gel Electrophoresis (PAGE), Agarose Gel Electrophoresis( AGE).• RAPD.• Gene Sequencing and analysis of the sequence using BLAST. ( familiar with Bioinformatics)• Cloning techniques . Plasmid extraction.• Cell cultureMicrobiology• Microscopy, Slide Study- differentiating different stages of the Malaria parasite, Cell counting and calculation of Parasitemia. • RBC & WBC counting using Haemocytometer and Sysmex.• Various Slide Staining techniques. • Synthesis of Hemozoin pigment from normal whole blood and infected blood samples. • Cell membrane purification.• Parasite Culturing in Whole Blood.• Plating techniques for growth and culturing of S. aureus to study drug effect on bacteria.• Spectroscopic studies of cell hemolysis to check effect of drug toxicity on RBCsI have conducted, coordinated & attended various workshops and Poster Presentations held in the Kuwait University. Show less

Tissue Culture• Maintaining Cell lines. • Plaque assay.• Transfection- Transactivation Assay.Molecular Biology• Western Blotting.• Plasmid DNA Extraction – Ligation, transformation, restriction digestion(single and double digest).• Cloning techniques. • Lymphocyte Extraction (using Ficoll).• Column Chromatography.• Poly-acrylamide Gel Electrophoresis (PAGE), Agarose Gel Electrophoresis( AGE).• PCR, RT-PCR.

Master'S Degree, Biotechnology

M.Sc. Biotechnology

Bachelor'S Degree, Chemistry

Education record